Relationship between serum expression levels of miR-214-3p and miR-124-3p and the severity of disease in patients with Parkinson's disease and their values in early diagnosis
10.11958/20250429
- VernacularTitle:帕金森病患者血清miR-214-3p、miR-124-3p表达水平与病情严重程度的关系及其早期诊断价值
- Author:
Meijuan ZHOU
1
;
Xiaoxiang MIAO
Author Information
1. 如皋市中医院急诊内科 226500
- Publication Type:Journal Article
- Keywords:
Parkinson disease;
microRNAs;
ROC curve;
severity;
miR-214-3p;
miR-124-3p
- From:
Tianjin Medical Journal
2025;53(5):498-502
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between the expression levels of serum microRNA-214-3p and miR-124-3p with the severity of Parkinson's disease(PD),and their early diagnostic value.Methods From January 2018 to December 2023,96 PD patients admitted to Rugao Traditional Chinese Medicine Hospital were selected as the PD group,and 106 healthy individuals were selected as the control group.According to the severity of 96 patients,they were grouped into the early stage group(I-II stage,n=59)and the mid to late stage group(III-V stage,n=37).RT-qPCR was applied to detect serum levels of miR-214-3p and miR-124-3p.Spearman method was used for correlation analysis.The influencing factors of PD severity were analyzed using multiple Logistic regression analysis.The efficacy of serum miR-214-3p and miR-124-3p in diagnosing PD and its severity was analyzed by receiver operating characteristics(ROC).Results Compared with the control group,the serum levels of miR-214-3p and miR-124-3p were lower in the PD group(P＜0.05).Compared with the early stage group,there were a longer disease course,higher level of homocysteine(Hcy)and lower levels of serum miR-214-3p and miR-124-3p in the mid to late stage group(P＜0.05).Spearman analysis showed that serum miR-214-3p and miR-124-3p were negatively correlated with Hoehn-Yahr staging in PD patients(P＜0.05).Multivariate Logistic regression showed that disease duration(OR=1.249),Hcy(OR=1.156),serum miR-214-3p(OR=0.925)and miR-124-3p(OR=0.917)were factors influencing the severity of PD(P＜0.05).ROC analysis showed that the sensitivity and specificity of serum miR-214-3p and miR-124-3p combined in the diagnosis of PD were 85.42%and 81.90%respectively.The sensitivity and specificity of serum miR-214-3p and miR-124-3p in the diagnosis of advanced PD were 89.16%and 79.66%respectively.Conclusion The serum levels of miR-214-3p and miR-124-3p are relatively low in PD patients,and their levels are related to the severity of disease.The detection of serum miR-214-3p and miR-124-3p is helpful for the diagnosis and evaluation of PD.
