Isolation,identification and biological characteristics of Aeromonas hydrophila from South China tiger
10.16303/j.cnki.1005-4545.2025.01.07
- VernacularTitle:华南虎源嗜水气单胞菌的分离鉴定及其生物学特性分析
- Author:
Yuqi LI
1
;
Yali KANG
;
Yubin ZHUO
;
Lingshan HUANG
;
Shuqi QIU
;
Yuxi XUE
;
Xiaop-ing WU
;
Sisi FAN
;
Yuting LIAO
;
Weiye LIN
;
Chan CHEN
;
Kaixiong LIN
;
Tengteng CHEN
;
Xipan LIN
;
Kewei FAN
Author Information
1. 福建农林大学动物科学学院,福建 福州 350002;龙岩学院生命科学学院福建省家畜传染病防治与生物技术重点实验室,福建龙岩 364012
- Publication Type:Journal Article
- Keywords:
South China tiger;
Aeromonas hydrophila;
isolation and identification;
biological charac-teristics
- From:
Chinese Journal of Veterinary Science
2025;45(1):46-52,58
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to identify the cause of death of a South China tiger cub at the Meihuashan breeding institute of Fujian Province.Pathogenic bacteria were isolated and cultured from liver,spleen,lung and other tissue samples of the dead South China tiger aseptically.The iso-lated bacteria were identified through morphological observation,biochemical characterization,sequence analysis of housekeeping gene gyrB,virulence gene detection,animal pathogenicity test and drug sensitivity test.A pathogenic Aeromonas hydrophila strain,designated FJ/Tiger-201809 was successfully isolated from the trachea of dead South China tiger.The nucleotide sequence ho-mology between the isolate and 11 strains of Aeromonas gyrB ranged from 91.2％to 99.1％,with the highest homology of 99.1％observed with Aeromonas hydrophila(AF208251.1).Genetic evo-lution analysis showed that the isolated strain FJ/Tiger-201809 was in the same evolutionary branch as other reference strains of Aeromonas hydrophila and was closely related.The pathoge-nicity test in mice showed artificial infection of mice with the strain resulted in varying degrees of lesions in several organs of the mice,and the median lethal dose(LD50)was 1 × 107.8 CFU/mL.Virulence gene test results showed that the isolate FJ/Tiger-201809 carried two virulence genes,aer and act.The results of drug sensitivity test showed that FJ/Tiger-201809 was highly sensitive to enrofloxacin and ampicillin among 18 commonly used antibiotics,relatively sensitive to penicil-lin G and doxycycline,and resistant to the other 14 antibiotics.In conclusion,this study isolated and identified a strain of Aeromonas hydrophila from a dead South China tiger with multiple drug resistance and strong pathogenicity,which provided an important reference for the prevention and control of bacterial diseases in South China tiger.
