PD-L1 monoclonal antibody enhances the effect of paclitaxel combined with lentinan against human breast cancer MDA-MB-231 in vitro
- VernacularTitle:PD-L1单抗加强紫杉醇联合香菇多糖体外抗人乳腺癌MDA-MB-231作用
- Author:
Fen LI
1
;
Nana PING
;
Jurong ZENG
;
Xiaoli XU
;
Peng LIU
Author Information
- Publication Type:Journal Article
- Keywords: programmed cell death-ligand 1(PD-L1)monoclonal antibody; paclitaxel(PTX); lentinan(LNT); anti-human breast cancer MDA-MB-231
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2025;46(1):94-100
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of programmed cell death-ligand 1(PD-L1)monoclonal antibody and paclitaxel(PTX)combined with lentinan(LNT)on human breast cancer MDA-MB-231 cells in vitro.Methods MDA-MB-231 cells,human peripheral blood mononuclear cells(PBMCs),and MDA-MB-231+PBMCs co-culture were randomly divided into control group,PTX group,LNT group,MPDL3280A(PD-L1 monoclonal antibody)group,PTX+LNT group,and PTX+LNT+MPDL3280A group.The cell activity was detected by CCK8.The expressions of MHC-I and PD-L1 were detected by flow cytometry.The levels of IFN-γ and TNF-α were detected by ELISA kits.Results Compared with control group,the activity of MDA-MB-231 cells was significant inhibited in PTX group,MPDL3280A group,PTX+LNT group,and PTX+LNT+MPDL3280A group(P＜0.01).LNT group and PTX+LNT+MPDL3280A group significantly promoted the immune effect of PBMCs(P＜0.05,P＜0.01).PTX+LNT+MPDL3280A group significantly inhibited the activity of MDA-MB-231cells co-cultured with PBMCs(0.56±0.16 vs.0.39±0.13,P＜0.05).Compared with the negative control,LNT significantly promoted the expression of PD-L1 in MDA-MB-231 and the secretion of IFN-γ by PBMCs(P＜0.05).Conclusion By blocking the effect between PD-L1 and PD-1,PD-L1 monoclonal antibody can improve immunity and promote the antitumor effect of PTX combined with LNT in vitro.
