Plasma-activated solutions alleviate DSS-induced colitis in mice and promote colonic epithelial cell repair through the eNOS pathway
- VernacularTitle:等离子体活化液通过eNOS途径缓解DSS诱导的小鼠结肠炎及结肠损伤
- Author:
Xueni WANG
1
;
Kaijie REN
;
Yuyi MA
;
Tianhao MIN
;
Xiaoyuan DENG
;
Yuanchang PENG
;
Yuanyuan LIU
;
Wei WANG
;
Tuanhe SUN
;
Chengxue DANG
;
Hao ZHANG
Author Information
- Publication Type:Journal Article
- Keywords: ulcerative colitis; plasma-activated solution(PAS); endothelial nitric oxide synthase
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2025;46(1):28-34
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the role and potential mechanisms of plasma-activated solution(PAS)in alleviating dextran sulfate sodium salt(DSS)-induced ulcerative colitis.Methods We constructed a DSS-induced ulcerative colitis mouse model and evaluated the effect of PAS in vivo by observing mouse weight,calculating disease activity indexes,detecting inflammatory factors and oxidative stress indicators through ELISA.We also evaluated the effect of PAS on colon cell proliferation and migration ability through clone formation experiments,scratch experiments,and used Western blotting to determine the expression levels of proliferation-related proteins.Results PAS significantly reversed DSS-induced weight loss and increased disease activity indexes in mice(P＜0.05).The serum inflammatory cytokine levels(TNF-α,IL-6 and IL-1β)in PAS group were significantly reduced compared to those in DSS group(P＜0.05).PAS treatment could improve the imbalance of colonic redox homeostasis including changes of malondialdehyde,catalase and superoxide dismutase caused by DSS(P＜0.05).After the use of endothelial nitric oxide synthase inhibitors,changes in various indicators caused by in vivo PAS disappeared(P＜0.001).The clone formation ability of colon cells was stronger in the group treated with PAS,and the expression of proliferation-related proteins increased.Cell scratch experiments suggested that intervention with PAS could reverse the decrease in cell migration ability caused by lipopolysaccharide(P＜0.001).After the application of endothelial nitric oxide synthase inhibitors,the pro-proliferative and migratory effects of PAS disappeared(P＜0.05).Conclusion PAS alleviate DSS-induced colitis in mice and promote colonic epithelial cell repair through the eNOS pathway.
