Baicalein intervenes in the Nrf-2/HO-1 signaling pathwayreduces kidney injury in sepsis mice
10.16303/j.cnki.1005-4545.2025.01.17
- VernacularTitle:黄芩素通过Nrf-2/HO-1信号通路改善脓毒血症小鼠肾损伤
- Author:
Yang HU
1
;
Chuanyi LYU
;
Xin DAI
;
Yuhang WANG
;
Ruizhu ZHAO
;
Jiaxuan FENG
;
Shil-ei LOU
;
Hui YAN
;
Cong SUN
Author Information
1. 长春中医药大学药学院,吉林长春 130117
- Publication Type:Journal Article
- Keywords:
baicalein;
antioxidant;
sepsis;
Nrf2;
mice
- From:
Chinese Journal of Veterinary Science
2025;45(1):121-128
- CountryChina
- Language:Chinese
-
Abstract:
Investigated the mechanisms by which baicalein regulates nuclear factor E2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)to ameliorate lipopolysaccharide(LPS)-induced sepsis-asso-ciated acute kidney injury(AKI)in mice.Sixty male C57BL/6 mice were randomly divided into six groups:control,model,low-dose baicalein(50 mg/kg),medium-dose baicalein(100 mg/kg),high-dose baicalein(200 mg/kg),and high-dose baicalein+brusatol(4 mg/kg).Baicalein was adminis-tered orally for 7 days as a preventative measure.Sepsis was induced via intraperitoneal injection of LPS.Murine sepsis score(MSS)was assessed within 12 hours post-induction.Serum creatinine(Scr),blood urea nitrogen(BUN),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),and interleukin-6(IL-6)were measured using an automatic biochemical analyzer and enzyme-linked immunosorbent assays(ELISA),respectively.Histopathological changes in kidney tissues were ob-served via hematoxylin-eosin(HE)staining.Western blot analysis was employed to determine the protein expression levels of Nrf2,HO-1,caspase-8,TNF-α,IL-1β,and IL-6 in kidney tissues.Additionally,total superoxide dismutase(SOD)activity in kidney tissues was assessed using com-mercially available kits.Compared to the model group,baicalein treatment significantly improved renal histopathological changes,alleviated cellular damage,and reduced the levels of inflammatory cytokines(TNF-α,IL-1βand IL-6)(P＜0.01)and kidney injury markers(Scr and BUN)(P＜0.01).Moreover,baicalein treatment significantly increased the protein expression levels of Nrf2 and HO-1(P＜0.01)and enhanced antioxidant enzyme activity.In conclusion,baicalein may pro-tect against LPS-induced sepsis-associated AK1 in mice by modulating the Nrf2/HO-1 signaling pathway,thereby attenuating oxidative stress,reducing inflammation,and disrupting the vicious cycle between inflammation and oxidative stress.
