The effects of lipopolysaccharide on proliferation and apoptosis of BESCs through the PI3K/AKT and Wnt/β-catenin signaling pathways
10.16303/j.cnki.1005-4545.2025.01.14
- VernacularTitle:脂多糖通过PI3K/AKT和Wnt/β-catenin信号通路对BESCs增殖和凋亡的影响
- Author:
Junsheng DONG
1
;
Zi WANG
1
;
Hanqing LI
1
;
Fangling ZHENG
1
;
Min ZHANG
1
;
Long GUO
1
;
Kangjun LIU
1
;
Luying CUI
1
;
Heng WANG
1
;
Jianji LI
1
Author Information
1. 扬州大学兽医学院江苏省动物重要疫病与人畜共患病防控协同创新中心,江苏扬州 225009;教育部农业与农产品安全国际合作联合实验室,江苏扬州 225009;江苏省高校动物重要疫病和重要人兽共患病防控技术国际合作联合实验室,江苏扬州 225009
- Publication Type:Journal Article
- Keywords:
BESCs;
LPS;
PI3K/AKT;
Wnt/β-catenin;
apoptosis;
proliferation
- From:
Chinese Journal of Veterinary Science
2025;45(1):100-106
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the effects of lipopolysaccharide(LPS)on the repair of bovine endo-metrial stromal cells(BESCs)during inflammatory response,BESCs were treated by LPS in this study.Cell apoptosis rate was detected using flow cytometry,cell viability was measured using the CCK-8 assay,cell migration ability was observed using a scratch assay,and the expression of con-nective tissue growth factor(CTGF),transforming growth factor-beta 3(TGF-β3)and vascular endothelial growth factor(VEGF)mRNA was measured using qRT-PCR.Additionally,the expression of key proteins in the PI3K/AKT and Wnt/β-catenin signaling pathways was assessed using Western blot analysis.The results showed that cell viability of BESCs significantly decreased(P＜0.01),cell migration ability decreased(P＜0.05),apoptosis rate of BESCs increased(P＜0.01),CTGF and TGF-β3 mRNA expression levels decreased(P＜0.01),while VEGF mRNA ex-pression increased after treatment with LPS(P＜0.01).The phosphorylation levels of PI3K,AKT and GSK-3β proteins decreased(P＜0.05),as well as the expression levels of c-Myc and Cyclin-D1 proteins also decreased(P＜0.01).These results indicated that LPS can inhibit the proliferation of BESCs and promote cell apoptosis possibly through the inhibition of the PI3K/AKT and Wnt/β-catenin signaling pathways.
