Identification of the Components of Yu-Ping-Feng-San Absorbed into Plasma Based on UPLC-Q-TOF-MS
10.14148/j.issn.1672-0482.2025.0637
- VernacularTitle:基于UPLC-Q-TOF-MS鉴定玉屏风散入血成分
- Author:
Fuman HUANG
1
;
Yajun SONG
;
Tongwen ZUO
;
Lin LI
;
Yunfeng ZHENG
;
Jie ZHENG
;
Min HONG
Author Information
1. 南京中医药大学药学院,江苏省中药药效与安全性评价重点实验室,江苏 南京 210023
- Publication Type:Journal Article
- Keywords:
Yu-Ping-Feng-San;
ethanol extract;
ultra-performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry;
component identification;
plasma-entering components;
Saposhnikoviae Radix;
Radix Astragali
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(5):637-651
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the plasma-entering components of the ethanol extract of Yu-Ping-Feng-San by ultra-per-formance liquid chromatography-quadrupole-time of flight tandem mass spectrometry(UPLC-Q-TOF-MS)technology,so as to pro-vide a reference for the study of the pharmacological substances of Yu-Ping-Feng-San.METHODS Male BALB/c mice were ga-vaged with the ethanol extract of Yu-Ping-Feng-San(6.5 g·kg-1),and plasma samples were collected before administration(0 h)and 1,2,4,6,8,and 12 h after administration.The UPLC-Q-TOF-MS technology was used to analyze and identify the components absorbed into the plasma.RESULTS A total of 73 chemical components in the ethanol extract of Yu-Ping-Feng-San were identi-fied,and 26 plasma-entering components were identified in all plasma samples.Among the plasma-entering components,11 compo-nents were from Saposhnikoviae Radix,and 15 components were from Radix Astragali.CONCLUSION The chemical components and plasma-entering components of the ethanol extract of Yu-Ping-Feng-San were analyzed comprehensively,which provides a reference for further clarifying the pharmacological substance basis and mechanism of action of Yu-Ping-Feng-San.
