Exploringinfection mechanism of mink enteritis virus by quantum dots-based sin-gle virus tracking
10.16303/j.cnki.1005-4545.2025.01.05
- VernacularTitle:利用量子点单病毒示踪技术研究水貂肠炎病毒的感染机制
- Author:
Yitong DONG
1
;
Xiaomeng WANG
;
Fengjiao YUE
;
Shujie WANG
;
Chunsheng WANG
Author Information
1. 东北林业大学生命科学学院,黑龙江哈尔滨 150040
- Publication Type:Journal Article
- Keywords:
mink enteritis virus;
quantum dots(QDs);
single virus tracking;
infection mechanism
- From:
Chinese Journal of Veterinary Science
2025;45(1):30-38
- CountryChina
- Language:Chinese
-
Abstract:
Quantum dot-based single-virus tracking has become a practical method to explore the molecular mechanism of virus entry into cells,because it can realize virion imaging in living cells.Mink enteritis virus(MEV)is a single-stranded DNA virus with strong environmental resistance,which has caused huge economic losses for the global mink farming industry.Although epidemio-logic and clinical aspects of MEVs have been studied,the mechanisms by which it infect target cells remain unclear.In this study,we implemented quantum dot labeling of mink enteritis virus u-sing the biotin-streptomycin affinity system and monitored the viral infection process in host cells.We found that MEV first attaches to the cell membrane and enters the cell by endocytosis.Live cell images showed quantum dot(QD)-MEV movement along microtubules,and viral infection was also inhibited by treatment with the microtubule inhibitor nocodazole,whereas the addition of the microfilament inhibitor CytoD had no effect on infection,suggesting that MEV transport is de-pendent on microtubules rather than microfilaments.Another imaging results showed that MEV co-localized with Rab5 and Rab7 in host cells,suggesting that the endosomal system is required for MEV internalization.Biochemical analyses showed that viral infection was significantly inhibited after pretreatment of host cells with the endosomal acidification inhibitors NH4 Cl and chloro-quine,suggesting that MEV invasion requires an acidic environment in the endosomes.Our results indicated that MEV enters early and late endosomes after entering the cell membrane,and that in-tracellular translocation is microtubule-dependent,which may in turn uncover a novel target for antiviral treatment.
