Establishment of competitive chemiluminescence method for detection of African swine fever virus p30 antibody
10.16303/j.cnki.1005-4545.2025.01.01
- VernacularTitle:非洲猪瘟病毒p30抗体竞争化学发光法的建立
- Author:
Shenghui WEN
1
;
Junjun SHAO
;
Shandian GAO
;
Decai PENG
;
Huiyun CHANG
;
Jiafeng DING
;
Wei LIU
;
Mingxian SHI
Author Information
1. 广西大学动物科学技术学院临床兽医学实验室,广西南宁 530004;中国农业科学院兰州兽医研究所动物疫病防控全国重点实验室/甘肃省病原生物学基础学科研究中心OIE/国家口蹄疫参考实验室,甘肃兰州 730046
- Publication Type:Journal Article
- Keywords:
African swine fever;
p30 antibody;
antibody detection;
chemiluminescence
- From:
Chinese Journal of Veterinary Science
2025;45(1):1-7
- CountryChina
- Language:Chinese
-
Abstract:
African swine fever(ASF)is an acute,febrile,and highly fatal disease caused by African swine fever virus(ASFV)in pigs.Given the current lack of commercial vaccines and the continu-ous evolution of ASFV in recent years,the emergence of moderately virulent genotype Ⅱ strains and the introduction of genotype Ⅰ attenuated strains have led to persistent and chronic infections in pigs.Therefore,the detection of specific antibodies against ASFV has become imperative.In this study,we established a competitive chemiluminescence immunoassay(p30-cCLIA)for detecting ASFV p30 antibodies using p30 monoclonal antibodies.By detecting sera with clear negative and positive backgrounds,we determined that the Cut-off value of this method was 50％,with both di-agnostic sensitivity(Dsn)and diagnostic specificity(Dsp)reaching 100％.Under optimal reaction conditions,we screened out an enzyme-labeled stabilizer suitable for p30 monoclonal antibody 16-5E7E8-HRP.Furthermore,the sensitivity of the established p30-cCLIA method was higher than that of the commercial blocking ELISA kit(1∶2 048 vs 1∶512)and exhibited good repeatability.Detection of sera positive for other porcine virus infections showed no cross-reactivity.The estab-lishment of this method provides a powerful tool for early diagnosis of ASF.
