Effect of zearalenone on proliferation and apoptosis of sika deer antler chondro-cytes
10.16303/j.cnki.1005-4545.2025.01.16
- VernacularTitle:玉米赤霉烯酮对梅花鹿鹿茸软骨细胞增殖与凋亡的影响
- Author:
Chenhao WANG
1
;
Xueyuan YAO
1
;
Baiyu LI
1
;
Qiaoling ZHANG
1
;
Zhanpeng YUE
1
;
Zhanqing YANG
1
;
Bin GUO
1
Author Information
1. 吉林大学动物医学学院,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
zearalenone;
deer antler;
chondrocytes;
proliferation;
apoptosis
- From:
Chinese Journal of Veterinary Science
2025;45(1):115-120,128
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of zearalenone(ZEA)on the proliferation and apoptosis of sika deer antler chondrocytes,the chondrocytes were isolated and cultured in vitro and treated with 50μmol/L ZEA for 24 h.Flow cytometry was used to assess cell proliferation,cell cycle,apoptosis,mitochondrial membrane potential,and intracellular levels of reactive oxygen species(ROS).The expression changes of hypertrophic cartilage cell marker genes Col X,Runx2,Alpl,and apoptosis-related genes Casp-3,Bax,Bcl-2 were measured using quantitative PCR.Additionally,glutathione reductase(GR)activity and the levels of the oxidative stress marker malondialdehyde(MDA)were determined.The results showed that after 24 h of ZEA treatment,cell proliferation was sig-nificantly inhibited,with an increase in the number of cells in the G0/G1 phase and a decrease in the S phase.The expression levels of hypertrophic chondrocyte marker genes Col X,Runx2 and Al-pl were significantly increased.Apoptosis rate was significantly increased,with elevated expression of pro-apoptotic genes Casp-3,Bax and reduced expression of the anti-apoptotic gene Bcl-2.The content of MDA in the antler chondrocytes increased,ROS levels rose,and GR activity decreased.The mitochondrial membrane potential reduced.The results suggested that ZEA could inhibit the proliferation of antler chondrocytes and promote the apoptosis by regulating cellular oxidative stress responses and the expression of apoptosis-related genes.
