Effects of prostaglandin D2 on cytokine secretion and phagocytosis and killing function in cow bone marrow-derived macrophages induced by E.coli
10.16303/j.cnki.1005-4545.2025.01.15
- VernacularTitle:前列腺素D2对大肠杆菌诱导的奶牛骨髓源巨噬细胞中细胞因子分泌及吞噬杀伤功能的影响
- Author:
Pengfei GONG
1
;
Xiaolin YANG
1
;
Lili GUO
1
;
Yu WANG
1
;
Jingze WU
1
;
Shangyi ZHANG
1
;
Bo LIU
1
;
Wei MAO
1
;
Jinshan CAO
1
Author Information
1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018;农业农村部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特 010018
- Publication Type:Journal Article
- Keywords:
E.coli;
prostaglandin D2;
cow bone marrow-derived macrophage;
pattern recognition re-ceptor;
phagocytosis
- From:
Chinese Journal of Veterinary Science
2025;45(1):107-114
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the effects of prostaglandin D2(PGD2)on cow bone marrow-derived macrophages induced by E.coli,cultured cow bone marrow-derived macrophages were taken as the research object.The effects of endogenous and exogenous PGD2 on the secretion and phagocytosis of E.coli induced proinflammatory cytokines(IL-1β,IL-6 and TNF-α)in macrophages were ana-lyzed.The results showed that the synthesis of PGD2 in macrophages induced by E.coli is depend-ent on the natural pattern recognition receptors TLR2,TLR4 and NLRP3.Inhibition of endogenous PGD2can down-regulate the secretion of pro-inflammatory cytokines(IL-1β,IL-6 and TNF-α)in E.coli induced macrophages(P＜0.001),and inhibition of endogenous PGD2 can enhance the kill-ing function of macrophages to a certain extent(P＜0.01).In addition,exogenous PGD2 could up-regulate the secretion of pro-inflammatory cytokines(IL-1β,IL-6 and TNF-α)in macrophages af-ter E.coli stimulation(P＜0.01),and exogenous PGD2 could weaken the killing function of mac-rophages within a certain concentration range(P＜0.01).Results indicated that PGD2 had certain effects on the secretion of cytokines and phagocytosis and killing function of macrophages induced by E.coli.
