Mechanism of modified Bushen Huoxue decoction in the treatment of lumbar disc herniation based on gene expression omnibus database and network pharmacology
10.3760/cma.j.cn431274-20241027-01615
- VernacularTitle:基于基因表达数据库与网络药理学研究补肾活血汤加减治疗腰椎间盘突出症的机制
- Author:
Yongli LIU
1
;
Litao LIU
1
;
Hualiang ZHU
1
;
Xuejian GOU
1
;
Xugang WU
1
;
Zongbo ZHOU
1
Author Information
1. 海口市中医医院骨伤科，海口 570216
- Publication Type:Journal Article
- Keywords:
Bushen Huoxue decoction;
Lumbar disc herniation;
Network pharmacology;
Gene expression omnibus
- From:
Journal of Chinese Physician
2025;27(8):1180-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential molecular mechanism of modified Bushen Huoxue decoction in the treatment of lumbar disc herniation (LDH) based on Gene Expression Omnibus (GEO) database and network pharmacology analysis.Methods:LDH samples were retrieved from GEO, and LDH targets were obtained from Gene Cards, CTD databases combined with GEO chips. The components and targets of modified Bushen Huoxue decoction were obtained by searching the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). STRING was used to draw the drug-component-target interaction network of modified Bushen Huoxue decoction in the treatment of LDH, so as to obtain the potential action targets of modified Bushen Huoxue decoction in the treatment of LDH. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were used to systematically analyze and elaborate the action targets.Results:A total of 23 LDH-related differential genes were identified after screening from GEO dataset GSE146904. A total of 652 potential targets of modified Bushen Huoxue Decoction were obtained from the database, and 45 were selected after screening. A total of 1 121 target genes related to LDH were obtained. By taking the intersection of the targets of modified Bushen Huoxue decoction and LDH disease targets, 8 potential targets of modified Bushen Huoxue decoction in the treatment of LDH were obtained, namely TNF, CASP3, PTGS2, ESR1, ACHE, BCL2, WNT3A, and CCL11. Modified Bushen Huoxue decoction in the treatment of LDH involves key processes such as regulation of metabolism and cell proliferation in the body; among them, cell nucleus, extracellular matrix, and mitochondria are important structures for the body to exert main functions; molecular functions mainly include protein and ion binding, cytokine activity, etc. KEGG pathway enrichment analysis results showed that classical WNT signaling pathway, cytokine signaling in immune system, interleukin-4/interleukin-13 signaling pathway, and apoptosis signaling pathway were key pathways.Conclusions:Modified Bushen Huoxue decoction can mediate immune and inflammatory responses of LDH through multiple targets and pathways, thereby improving LDH symptoms.
