Effect of ultrasound treated polystyrene nanoplastics on lipid accumulation in macro-phages
10.20039/j.cnki.1007-3949.2025.06.006
- VernacularTitle:超声处理的聚苯乙烯纳米塑料对巨噬细胞脂质蓄积的影响
- Author:
Hui LIANG
1
;
Lei WANG
;
Peiqing LIU
;
Yang YU
Author Information
1. 山东第一医科大学(山东省医学科学院)药学院(药物研究所),山东省 济南市 250117
- Publication Type:Journal Article
- Keywords:
polystyrene nanoplastics;
macrophage;
lipid accumulation;
high-frequency ultrasound
- From:
Chinese Journal of Arteriosclerosis
2025;33(6):500-506
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of ultrasound-treated polystyrene nanoplastics(PS-NP)on lipid accumu-lation in macrophage-derived foam cells.Methods The CCK-8 method was used to detect the effects of PS-NP and high-frequency ultrasound-treated PS-NP(UPS-NP)on the activity of macrophages,oil red O staining and cholesterol de-tection kit were used to detect the intracellular lipid accumulation,RT-qPCR and Western blot were used to detect the ex-pression of mRNA and protein related to cholesterol uptake and efflux,as well as mRNA levels of cell burial related recep-tors.Results UPS-NP had no significant effect on the activity of macrophages,but UPS-NP could significantly in-crease the formation of macrophage-derived foam cells and increase the lipid accumulation in foam cells.UPS-NP could significantly upregulate the mRNA and protein expression of CD36 and scavenger receptor-A1(SR-A1),but did not affect the mRNA and protein expression of cholesterol efflux related receptors ATP-binding cassette transporter A1(ABCA1)and scavenger receptor-B1(SR-B1).UPS-NP did not affect the mRNA levels of receptors related to cell burial processes.Conclusion Ultrasound-treated PS-NP can significantly increase lipid accumulation in macrophage-derived foam cells,and its mechanism is related to the upregulation of CD36 and SR-A1 expression.
