Metagenomic analysis of the intervention effect of aerobic exercise and complex polysaccharide supplementation on gut microbiota in high-fat diet induced obese rats
10.3969/j.issn.1000-6710.2024.12.006
- VernacularTitle:有氧运动与补充复合多糖对高脂膳食诱导肥胖大鼠肠道菌群干预效果的宏基因组学分析
- Author:
Yuxing ZHOU
1
;
Chenjing QI
;
Tingting LI
;
Jingwen GONG
;
Tingting XIE
;
Zhuang GUO
;
Jibin SHA
Author Information
1. 山东体育学院研究生教育学院 济南 250102
- Publication Type:Journal Article
- Keywords:
aerobic exercise;
obesity;
compound polysaccharides;
gut microbiota;
metagenomics
- From:
Chinese Journal of Sports Medicine
2024;43(12):981-995
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the different intervention effect of aerobic exercise or complex poly-saccharide supplement on gut microbiota(GM),GM-related metabolic pathway genes,as well as fat and weight losing of obese rats induced by high-fat diet. Methods Thirty-two male Sprague-Dawley rats were randomly divided into a control group,an obesity control(OC) group,an aerobic exercise (AE) group and a complex polysaccharide intervention(CPI) group,each of 8. The AE group was giv-en moderate intensity treadmill aerobic training for 6 weeks,while the CPI group underwent plant poly-saccharide gavage for the same period of time. Morphological indicators were measured and recorded,serum regulators of lipid metabolism were collected,and feces were collected for metagenomic sequenc-ing and analysis. Results(1) Before the intervention,the body weight of group OC was significantly higher than the control group(P<0.01). However,after 6-week aerobic exercise,the body weight and Lee's index of group OC reduced significantly(P<0.05). (2) The concentrations of peroxisome prolifera-tor-activated receptor γ(PPAR-γ) and leptin(LEP) in serum of the OC group were significantly high-er than the control group(P<0.001),but that of lipoprotein lipase(LPL) was significantly lower than the control group(P<0.001). The serum concentrations of PPAR-γ in group AE were significantly low-er than group OC(P<0.01),while those of PPAR-γ and LEP in group CPI were significantly lower than group OC(P<0.001). (3) The abundance of Akkermansia mucinphila,Alistipes finegoldii,Bacteroides ovatus and Gram-negative bacterium cL10-2b-4 in group OC was significantly lower than the control group(P<0.05),with that of Prevotella copri in group AE significantly higher than group OC and that of Helicobacter rodentium significantly lower than the latter(P<0.05). Moreover,the abundance of Bifido-bacterium animalis in group CPI was significantly higher than group OC,while that of Alistipes shahii was significantly lower than the latter(P<0.05). (4) Compared with the control group,the obese group was significantly enriched in Vibrio cholerae pathogenic cycle,Staphylococcus aureus infection and vari-ous types of N-glycan biosynthesis related genes. Moreover,the abundance of salmonella infection and N-glycan biosynthesis-related genes in group AE was significantly lower than group OC(P<0.05),and that of Vibrio cholerae disease-cycle related genes in group CPI was significantly lower than group OC (P<0.05). Conclusion The imbalance in the gut microbiota structure,and the abnormal expression of related metabolic pathway genes both play important roles in the phenotype changes of high-fat diet in-duced rats,which can be effectively relieved by aerobic exercise and complex polysaccharide gavage. However,there are obvious differences in the effects and possible mechanisms between the above two intervention methods.
