Effect of Salidroside on Endoplasmic Reticulum Stress and Connexin 43 in Rats with Myocardial Ischemia Reperfusion Injury
10.3870/j.issn.1004-0781.2025.03.004
- VernacularTitle:红景天苷对心肌缺血-再灌注损伤大鼠内质网应激与Cx43的作用
- Author:
Yuanheng HUANG
1
;
Yang HE
;
Xiaoliang HUANG
;
Xue WEI
;
Yaosheng WU
;
Yingxin LI
Author Information
1. 广西医科大学基础医学院,南宁 530021
- Publication Type:Journal Article
- Keywords:
Salidroside;
Ischemia-reperfusion;
Endoplasmic reticulum stress;
Apoptosis;
Gap junction protein
- From:
Herald of Medicine
2025;44(3):366-371
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of salidroside(Sal)on endoplasmic reticulum stress and connexin 43 in rats with myocardial ischemia-reperfusion injury(MIRI).Methods SD rats were randomly divided into Sham group,MIRI group,low-does Sal(Sal-L)group and high-does Sal(Sal-H)group.The Sham group and MIRI group were intraperitoneal injec-ted with 0.9％sodium chloride solution(10 mL·kg-1·d-1),the Sal-L group and Sal-H group were intraperitoneal injected at a volume of 10 mL·kg-1 with Sal(12,36 mg·kg-1·d-1),respectively.Each group was given a corresponding intervention once a day for 3 d.The MIRI model was established 30 min after the last administration in all groups except the Sham group.The patho-logical changes of myocardial tissue were observed by Hematoxylin-eosin(HE)staining.TdT-mediated-dUTP nick end labeling(TUNEL)was used to observe the apoptosis of cardiomyocyte,the genes and proteins expression of Cx43 and endoplasmic reticu-lum stress related factors such as GRP78,Caspasel2,CHOP and so on were detected by quantitative real-time polymerase chain reaction(q-PCR)and western blot analysis.Results Compared with the MIRI group,the degree of tissue and cell injury in each Sal group was alleviated,with a decreased apoptosis rate observed in the Sal-H group(P＜0.05),the gene expression of Cx43 was up-regulated while GRP78,Caspase12,and CHOP gene expressions were down-regulated in both does groups of Sal.The protein expressions of Cx43 and GRP78 were also be up-regulated and down-regulated respectively in both dose groups of Sal,meanwhile the protein expressions of CHOP,Bax,Caspasel2 and cleaved-Caspase3 were down-regulated and the protein expres-sion of Bcl-2 was up-regulated in SAL-H group(P＜0.05).Conclusion The protective effect of salidroside on cardiomyocytes may be related to the inhibition of endoplasmic reticulum stress-induced apoptosis and the imbalance of Cx43 metabolism.
