Effect of Nf1 gene silencing on proliferation and migration and phenotypic transfor-mation of mouse aortic vascular smooth muscle cells
10.20039/j.cnki.1007-3949.2025.06.005
- VernacularTitle:Nf1基因沉默对小鼠主动脉血管平滑肌细胞增殖与迁移及表型转化的影响
- Author:
Yang YANG
1
;
Zhichao YAO
1
;
Lirong HUO
1
Author Information
1. 首都医科大学附属复兴医院中心实验室,北京市 100038
- Publication Type:Journal Article
- Keywords:
vascular smooth muscle cell;
cell proliferation;
cell migration;
phenotypic transformation;
Nf1 gene
- From:
Chinese Journal of Arteriosclerosis
2025;33(6):493-499
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of Nf1 gene silencing on smooth muscle cell proliferation and migration and its possible molecular mechanism.Methods Using mouse aortic vascular smooth muscle cells(MOVAS)as the research object,the efficiency of Nf1 siRNA transfection in MOVAS was verified by RT-qPCR after transfection with Nf1 siRNA.The experiment was divided into control group,siRNA NC group,and Nf1 siRNA group.Ki-67 immunofluores-cence assay was used to detect the proliferation of MOVAS.Scratch assay and Transwell assay were used to evaluate the effect in the migration ability of MOVAS caused by Nf1 gene silencing.Western blot was used to detect the effect of Nf1 gene silencing on the expression levels of MOVAS phenotype marker proteins and the differences in the expression levels of downstream Ras signals extracellular signal-regulated kinase(ERK),p-ERK,protein kinase B(Akt),and p-Akt.Results Compared with the control group,the proliferation ability of MOVAS in the Nf1 siRNA group was significantly in-creased(increased by 33.23%,P<0.05),migration ability was significantly enhanced(scratch assay showed an increase of35.47%,Transwell assay showed an increase of 39.33%,P<0.05 or P<0.01),and the expression of contractile pro-teins smooth muscle 22α(SM22α),α-smooth muscle actin(α-SMA),and calmodulin 1(CNN1)was reduced(de-creased by 18.91%,23.38%and 25.08%,P<0.05 or P<0.01,respectively).The expression of synthetic protein os-teopontin(OPN)was increased(increased by58.70%,P<0.05),and the levels of p-ERK and p-Akt were significantly increased(increased by33.30%and2.42 times,respectively,P<0.05 or P<0.01).Conclusion Nf1 gene silen-cing can significantly enhance the proliferation and migration ability of MOVAS,and transform from a contractile phenotype to a synthetic phenotype.
