Application of Cytb and 12S rRNA in wildlife species identification for forensic science
10.13618/j.issn.1001-5728.2025.03.008
- VernacularTitle:Cytb和12S rRNA基因在法医学野生动物种属鉴定中的应用
- Author:
Dezhi JIANG
1
;
Yaxin ZHANG
;
Yu ZANG
;
Maolei AN
;
Zan ZHANG
;
Chengcheng QIU
;
Yaoheng JIANG
;
Wei SONG
;
Hong ZHAO
;
Kun XIE
;
Jiayi CHEN
;
Riga WU
Author Information
1. 青岛市公安局刑警支队,山东青岛 266000
- Publication Type:Journal Article
- Keywords:
Species identification;
DNA barcode;
Cytb;
12S rRNA;
Wildlife
- From:
Chinese Journal of Forensic Medicine
2025;40(3):308-311,322
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and compare the efficacy of DNA barcode,i.e.,Cytochrome b(Cytb)and 12S ribosomal RNA(12S rRNA)gene sequences,in the species identification of wildlife.Methods DNA extraction,quantification,PCR amplification of Cytb and 12S rRNA gene fragments,Sanger sequencing,and sequence alignment analysis were performed on ten wildlife samples.Results Both gene fragments were successfully amplified in six samples,while Cytb alone was successfully amplified in 1 sample,and 12S rRNA alone in 3 samples.Sequence analysis indicated that Cytb enabled species-level identification for 6 samples(Gallinula chloropus,Streptopelia orientalis,Phasianus colchicus,Falco naumanni,Myiopsitta monachus and Lynx lynx)and genus-level identification for 1 sample(Lepus).In contrast,12S rRNA achieved species-level identificaggion for 8 samples(Gallinula chloropus,Lepus sinensis,Phasianus colchicus,Myiopsitta monachus,Muntiacus reevesi,Macaca mulatta and Lynx lynx),representing seven species,and genus-level identification for 1 sample(Falco).However,by combining Cytb and 12S rRNA,all samples could be identified to the species level.Conclusion When applying DNA barcodes to wildlife identification,the Cytb and 12S rRNA gene regions analyzed here can effectively identify common species such as Gallinula chloropus and Streptopelia orientalis,but face difficulties in distinguishing closely related species within the same genus.Therefore,when conducting wildlife species identification,it is recommended to use two or more DNA barcode markers.
