Detection of etomidate,metomidate,propoxate,and isopropoxate in human hair by UPLC-MS/MS
10.13618/j.issn.1001-5728.2025.03.002
- VernacularTitle:UPLC-MS/MS法检测人体毛发中依托咪酯、美托咪酯、丙帕酯和异丙帕酯
- Author:
Xingang MIAO
1
;
Limei LU
;
Mingyang JIN
;
Yuting HUANG
;
Yuyan CAI
;
Yu ZOU
;
Kejun ZHANG
;
Qihua CHEN
Author Information
1. 广东华生司法鉴定中心,广东 广州 510410
- Publication Type:Journal Article
- Keywords:
Forensic toxicology;
Ultra performance liquid chromatography(UPLC-MS);
Etomidate;
Metomidate;
Propoxate;
Isopropoxate;
Hair
- From:
Chinese Journal of Forensic Medicine
2025;40(3):273-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a rapid and accurate ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)method for the detection of etomidate,metomidate,propoxate,and isopropoxate in human hair.Methods Hair samples containing etomidate,metomidate,propoxate,and isopropoxate were extracted with methanol containing the internal standard orthoxine,filtered with a 0.22 μm organic filter membrane and detected vio UPLC-MS/MS.All components were separated by using a gradient elution procedure consisting of 0.01％formic acid(1 mmol/L ammonium acetate)and acetonitrile.Positive electrospray ionization was performed using multiple reaction monitoring(MRM)mode.Results The linear relationships of etomidate,metomidate,propoxate,and isopropoxate were good in the range of 0.01～1 ng/mg(r ≥ 0.997 9),with recovery rates ranging from 87.9％to 101.5％.The accuracy was between 80.0％and 110.0％.The intra-day and inter-day relative standard deviations(RSD)were 2.9％～9.6％and 3.6％～19.9％.Conclusion This method is easy to operate and has high recovery efficiencies.It is sufficiently simple and sensitive to be applied to detect etomidate,metomidate,propoxate,and isopropoxate in hair.
