Effects of Esketamine on the proliferation,migration,and invasion of liver cancer cells by regulating the Wnt/β-catenin signaling pathway
10.3969/j.issn.1005-6483.20240083
- VernacularTitle:艾司氯胺酮调节Wnt/β-catenin信号通路对肝癌细胞增殖、迁移和侵袭的影响
- Author:
Zhenyun WANG
1
;
Hongtao WANG
1
;
Ruixin ZHOU
1
;
Jinguang ZHANG
1
Author Information
1. 061000 河北省沧州中西医结合医院麻醉科
- Publication Type:Journal Article
- Keywords:
esketamine;
Wnt/β-catenin signaling pathway;
liver cancer cells;
proliferation;
migration;
invasion
- From:
Journal of Clinical Surgery
2025;33(4):433-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Esketamine(S-KET)on the proliferation,migration,invasion,and Wnt/β-catenin signaling pathway of liver cancer cells.Methods Hep G2 liver cancer cells were separated into Control group,low,medium,and high concentration treatment groups(S-KET-L,S-KET-M,S-KET-H groups),and high concentration treatment group(S-KET-H+LiCl group);Edu was applied to detect cell proliferation;scratch experiment was applied to detect cell migration;Transwell experiment was applied to detect cell invasion.Western blot was applied to detect the expression of nuclear proliferation antigen markers(Ki-67),Cyclin D1,matrix metalloproteinase 2(MMP-2),matrix metalloproteinase 9(MMP-9),Wnt1,and β-catenin proteins;nude mouse transplanted tumor was applied to detect the effect of Esketamine on the growth of liver cancer transplanted tumors;immunohistochemistry was applied to detect the expression of Wnt1 and β-catenin proteins.Results Compared with the Control group,the Edu positive rate,Ki-67,Cyclin D1,scratch healing rate,number of cell invasions,the expression of MMP-2,MMP-9,Wnt1,and β-catenin proteins in Hep G2 cells in the S-KET-L,S-KET-M,and S-KET-H groups were obviously reduced(P＜0.05);compared with the S-KET-H group,the Edu positive rate,Ki-67,Cyclin D1,scratch healing rate,number of cell invasions,the expression of MMP-2,MMP-9,Wnt1,and β-catenin proteins in Hep G2 cells in Hep G2 cells in the S-KET-H+LiCl group were significantly increased(P＜0.05);the results of nude mouse tumor transplantation experiment showed that compared with the Control group,the S-KET group mice showed slow tumor growth,reduced tumor mass and volume,and significantly reduced expression of Wnt1 and β-catenin proteins(P＜0.05).Conclusion Esketamine can inhibit the proliferation,migration,and invasion of liver cancer cells by inhibiting the Wnt/β-catenin signaling pathway.
