Discrimination of half-sibling relationship using diverse reference samples
10.13618/j.issn.1001-5728.2025.03.020
- VernacularTitle:引入不同参考样本甄别半同胞关系
- Author:
Haixia LI
1
;
Yuluo LIU
;
Xuegang LI
;
Huidan LIAO
Author Information
1. 广东警官学院刑事技术学院,广东 广州 510440
- Publication Type:Journal Article
- Keywords:
Forensic genetics;
Half-sibling testing;
Half-sibling index;
Family reconstruction
- From:
Chinese Journal of Forensic Medicine
2025;40(3):369-372,374
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze 25 cases of the half-sibling identification involving two mothers(same father,different mothers)by using family reconstruction method.Methods Hypothesis testing for half-sibling relationships were established using likelihood ratio methods.The family reconstruction was performed according to the rule of Mendelian inheritance.HSI values were calculated for dyads(two individuals),triads(three individuals with one biological mother),and tetrads(four individuals with two biological mothers)along with unrelated control groups,using 23 and 41 autosomal STR markers.Results Compared to the dyadic and triadic groups,the tetrad group exhibited a significant increase in HSI values(102～105),enabling clear discrimination of half-sibling relationships.Overlapping areas decreased,from 20.29％to 0.83％,which was reduced by 24 times,indicating enhanced system efficacy.Conclusion Incorporating additional reference samples is a feasible strategy to improve the efficacy of half-sibling identification under constrained analytical conditions.The approach enhances the detection reliability and offers valueable insights for forensic genetic practice.
