Progress and application of lyophilization technology in forensic DNA testing
10.13618/j.issn.1001-5728.2025.03.014
- VernacularTitle:冻干技术在法医DNA检验中的研究进展及应用
- Author:
Yuling CUI
1
;
Hao YU
;
Zhe ZHANG
;
Lina JIA
;
Jing WANG
;
Xue BAI
Author Information
1. 河北医科大学,河北石家庄 050017;公安部鉴定中心,北京 100038
- Publication Type:Journal Article
- Keywords:
Forensic genetics;
Freeze-drying technology;
PCR;
Lyoprotectants;
Trace DNA samples
- From:
Chinese Journal of Forensic Medicine
2025;40(3):343-347
- CountryChina
- Language:Chinese
-
Abstract:
Freeze-drying technique,also known as lyophilization,is a process of removing moisture from a solution or suspension through freezing and vacuum dehydration to maintain the stability of the samples and prolong their shelf life.Freeze-drying technology has been widely used in food,pharmaceutical,clinical testing,chemical and other fields but its application in the field of forensic medicine has just started.Polymerase chain reaction(PCR)and its derivative detection technologies are widely used in forensic DNA detection,but PCR reagents need to be stored and transported at low temperature.In recent years,forensic scientists have begun applying freeze-drying technology to PCR amplification reagents to solve the transportation and storage problems of PCR reagents.In order to promote the application of PCR freeze-drying technology in forensic genetics,this paper mainly expounds the research course,system and process of PCR freeze-drying technology,compares the advantages and disadvantages of PCR reagents with traditional PCR reagents,and introduces the advantages and challenges of PCR freeze-drying reagents in forensic medicine.
