Influencing factors on the success rate of patient-derived gastrointestinal malignant tumor organoid culture
10.3760/cma.j.cn113855-20250407-00210
- VernacularTitle:患者来源胃肠道恶性肿瘤类器官培养成功率的影响因素分析
- Author:
Qian LIU
1
;
Yajing LIU
;
Sihan ZHAO
;
Yuqi SUN
;
Zequn LI
;
Xiaodong LIU
;
Yulong TIAN
;
Shougen CAO
;
Pu CHEN
;
Yanbing ZHOU
Author Information
1. 青岛大学附属医院胃肠外科，青岛 266000
- Publication Type:Journal Article
- Keywords:
Organ culture technique;
Stomach neoplasms;
Colorectal neoplasms;
Bioartificial organs
- From:
Chinese Journal of General Surgery
2025;40(6):473-480
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the factors influencing the success rate of culturing patient-derived gastric and colorectal cancer organoids.Methods:From Feb 2022 to Oct 2023, 398 tumor tissue specimens from patients who underwent gastric cancer and colorectal cancer resection at the Department of Gastrointestinal Surgery, the Affiliated Hospital of Qingdao University, were used for organoid culture. The clinicopathological factors affecting the success rate of organoid culture were analyzed.Results:The overall success rate of organoid culture in this group was 75.1% (299/398), with the success rate of gastric cancer organoid culture being 79.8%(154/193) and that of colorectal cancer being 70.7% (145/205). Different clinicopathological T stage ( χ2=4.765, P<0.05),histological type ( χ2=11.248, P<0.05), and tumor regression grade (TRG) grade after neoadjuvant chemotherapy ( χ2=7.797, P<0.05) were related to the success rate of organoid culture . Multivariate analysis showed that the TRG grade was an independent influencing factor( P=0.040). For colorectal cancer, different pathological T stage ( χ2=5.108, P<0.05), histological type ( χ2=11.270, P<0.05), and TRG grade after neoadjuvant chemotherapy ( χ2=6.797, P<0.05) were related to the success rate of organoidculture . Different from gastric cancer, the results of multivariate analysis of colorectal cancer showed that the histological type was an independent influencing factor ( P=0.018). Conclusions:The pathologic T stage, histological type of tumors, and TRG of cancer patients all have a significant impact on the success rate of establishing tumor organoids. Among them, the TRG grade is an independent influencing factor for the culture of gastric cancer organoids, and the histological type is an independent influencing factor for colorectal cancer organoids.
