A study on the effect and mechanism of new 6449Da active peptide in regulating the apoptosis and proliferation of gastric adenocarcinoma cells
10.3760/cma.j.cn113855-20240530-00396
- VernacularTitle:新6449Da活性肽调控胃腺癌细胞凋亡和增殖的作用及其机制研究
- Author:
Dongjian SONG
1
;
Qi WANG
;
Meng SU
;
Qiuliang LIU
;
Yingzhong FAN
;
Heying YANG
;
Qian ZHANG
;
Da ZHANG
Author Information
1. 郑州大学第一附属医院小儿外科, 郑州 450000
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Apoptosis;
Cell proliferation;
Active peptide
- From:
Chinese Journal of General Surgery
2025;40(5):392-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the biological efficacy and molecular mechanism of the new 6449Da active peptide against gastric adenocarcinoma.Methods:Effect 6449Da active peptides on SGC-7901 gastric adenocarcinoma cells line was studied and mRNA and protein expression levels of key factors relating to apoptosis, proliferation and notch signaling pathway was detcted.Results:The 6449Da functional fragment WSGC peptide located on the cell membrane relatively specifically inhibited the growth of gastric adenocarcinoma cells, but had a weak effect on normal gastric mucosal cells [cell doubling time , SGC-7901, 1.2 μmol/ml: (38.22±6.80) h vs. 0 μmol/ml: (25.65±1.82) h, P<0.05; GES-1, 1.2 μmol/ml: (33.37±3.15) h vs. 0 μmol/ml: (29.25±1.26) h, P>0.05].After treatment of SGC-7901 cells by IC80 (48 h),the cell apoptosis rate was higher than that in the control group (IC80: 0.421±0.036 vs. control group: 0.028±0.004, P<0.001), while the cell proliferation coefficient,number of transmembrane invasive cells and relative migration rate were all lower than those of the control group (all P<0.01).Compared with the control group, the mRNA and protein expression levels of Bax and Caspase-3 were higher than those in the control group(all P<0.01).Compared with the control group, the mRNA and protein expression levels of Ki67, PCNA, NOTCH1,Jagged 1, MAML3, CSL and HES1 were significantly lower than those in the control group (all P<0.01).After treatment with WSGC bioactive peptides, tumor weight was significantly lower than that of the control group [40 nmol/g: (0.463±0.031) g, 80 nmol/g: (0.340±0.040) g vs. control group: (1.667±0.373) g, all P<0.01]. Conclusion:6449Da functional fragment WSGC active peptide relatively specifically interferes with the growth of gastric adenocarcinoma cells by inhibiting the Notch signaling pathway, and has the biological effects of promoting apoptosis,inhibiting proliferation, transmembrane invasion and migration.
