Expression of Cullin-associated NEDD8-dissociated protein 1 in colorectal cancer and its clinical significance
10.3760/cma.j.cn113855-20231228-00446
- VernacularTitle:Cullin相关NEDD8解离蛋白1在结直肠癌中的表达及其临床意义
- Author:
Changjiang YANG
1
;
Long ZHAO
1
;
Yilin LIN
1
;
Yingjiang YE
1
;
Shan WANG
1
;
Zhanlong SHEN
1
Author Information
1. 北京大学人民医院胃肠外科 北京大学人民医院外科肿瘤研究室 北京市结直肠癌诊疗研究重点实验室，北京 100044
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Cullin proteins;
Neoplasm staging
- From:
Chinese Journal of General Surgery
2025;40(7):558-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of Cullin associated NEDD8 dissociated protein 1 in colorectal cancer and its effect on the biological behavior of colorectal cancer.Method:A total of 70 pairs of colorectal cancer and paired normal tissue specimens were collected from Jun to Dec 2017 at the Department of Gastrointestinal Surgery at Peking University People's Hospital. Immunohistochemistry was used to detect the expression of Cullin associated NEDD8 dissociated protein 1 and analyze its relationship with clinical pathological indicators and prognosis. CCK8, colony formation assay, transwell assay, and wound healing assay were used to evaluate the effects of Cullin associated NEDD8 dissociated protein 1 on the proliferation, migration, and invasion ability of colon cancer cells.Result:Compared with normal tissues, Cullin associated NEDD8 dissociated protein 1 was highly expressed in colorectal cancer (Immunohistochemistry score: 3.685±1.257 vs. 2.000±0.851, Z=6.536, P<0.001). The expression level of Cullin associated NEDD8 dissociated protein 1 was significantly correlated with T stage ( χ2=5.67, P=0.017), N stage ( χ2=7.20, P=0.007), and pathology stage ( χ2=4.66, P=0.031). Patients with high expression of Cullin associated NEDD8 dissociated protein 1 had a worse prognosis than those with low expression ( χ2=4.80, P=0.037). Knocking down Cullin associated NEDD8 dissociated protein 1 significantly reduced the proliferation, colony formation, and invasive migration ability of DLD1 cells (all P<0.05). Conclusion:Cullin associated NEDD8 dissociated protein 1 is significantly overexpressed in colorectal cancer and has a promoting effect on the occurrence and development of colorectal cancer.
