Predictive Value of Serum IRF4 and RANKL Levels for Recurrence and Correlation with other Immunological Indicators in Children with Recurrent Oral Ulcers after Treatment
10.3969/j.issn.1671-7414.2025.01.021
- VernacularTitle:复发性口腔溃疡患儿血清IRF4,RANKL水平与其他免疫学指标相关性及对再复发的预测价值
- Author:
Qi ZHANG
1
;
Huawei LIU
Author Information
1. 首都医科大学附属北京朝阳医院口腔科,北京 100020
- Publication Type:Journal Article
- Keywords:
recurrent oral ulcers;
interferon regulatory factor 4;
receptor activator of nuclear factor-κB ligand
- From:
Journal of Modern Laboratory Medicine
2025;40(1):110-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the predictive value of serum interferon regulatory factor 4 (IRF4) and receptor activator of nuclear factor-κB ligand (RANKL) levels for recurrence and correlation with other immunological indicators in children with recurrent oral ulcers after treatment. Methods Eighty children with recurrent oral ulcers admitted to Beijing Chaoyang Hospital,Capital Medical University from January 2019 to June 2022 were collected as the diseased group. They were separated into a nonrecurrent group (n=61) and a recurrent group (n=19) based on follow-up results. Additionally,49 oral health volunteers who underwent physical examinations were regarded as the control group. ELISA was applied to detect serum levels of IRF4 and RANKL. General clinical data of patients were collected and analyzed. Multivariate logistic regression was applied to analyze the factors affecting the recurrence of children with recurrent oral ulcers. The Pearson method was applied to analyze the correlation between IRF4,RANKL,and immunological indicators. The receiver operating characteristic (ROC) curve was plotted to analyze the predictive value of serum IRF4 and RANKL levels for recurrence in children with recurrent oral ulcers after treatment. Results Compared with the control group,the levels of IRF4(9.67±1.03ng/ml,7.86±0.92ng/ml vs 6.19±0.71ng/ml) and RANK(192.95±19.86pg/ml,152.56±15.98pg/ml vs 83.96±9.85pg/ml) in the non recurrent group and the recurrent group,compared with the non recurrent group,the levels of IRF4 and RANKL in the recurrent group were increased,and the differences were statistically significant (F=121.514,487.250,all P<0.05). There were statistical difference between the two groups in terms of inflammatory factors such as IL-1β,TNF-α,IL-6 and CD4+,CD4+/CD8+(t=6.926～15.648,all P<0.05). Logistic regression analysis,IRF4[OR(95％CI):1.529(1.079～2.167)],RANKL[OR(95％CI):1.421(1.049～1.925)]and IL-1β[OR(95％CI):1.322(1.007～1.736)]were all risk factors for recurrence in children with recurrent oral ulcers ( all P<0.05),while CD4+[OR(95％CI):0.788(0.641～0.968)]was protective factor for recurrence in children with recurrentoral ulcers (P<0.05). Pearson correlation analysis,IRF4,RANKL were positively correlated with inflamnatory factors (r=0.453～0.533,all P<0.05). CD4+(r=-0.407,-0.409) and CD4+/CD8+(r=-0.425,-0.412) were positively correlated with inflammatory factors (P<0.05),ROC curve results revealed that the AUC(95％CI)of serum IRF4,RANK,and their combination in predicting recurrence in children with recurrent oral ulcers was 0.840(0.741～0.913),0.832(0.732～0.906) and 0.928(0.847～0.974) respectively. The combined prediction was better than that of IRF4 and RANKL alone prediction(Z=1.984,2.071,P=0.047,0.038). Conclusion The serum levels of IRF4 and RANKL in children with recurrent oral ulcers obviously increase,both of which are risk factors affecting recurrence in children after treatment,and have certain auxiliary predictive values for recurrence in children after treatment.
