Establishment and evaluation of rat model of type 2 diabetes with liver fibrosis
10.3969/j.issn.1005-4847.2025.01.008
- VernacularTitle:2型糖尿病合并肝纤维化大鼠模型构建的初探
- Author:
Hongxing HE
1
;
Naishun LIAO
Author Information
1. 福建医科大学实验动物中心,福州 350004
- Publication Type:Journal Article
- Keywords:
type 2 diabetes mellitus;
liver fibrosis;
mellitus with liver fibrosis;
rats
- From:
Acta Laboratorium Animalis Scientia Sinica
2025;33(1):82-88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rat model of type 2 diabetes mellitus with hepatic fibrosis using a high-lipid-sugar diet(HLSD)combined with inducement with carbon tetrachloride(CCl4)and streptozotocin.Methods SD rats were randomly divided into a liver fibrosis(LF)group,type 2 diabetes combined with liver fibrosis(DLF)group,and normal group,with 10 rats in each group.All rats in the LF or DLE group were fed an HLSD for 4 weeks;then,all rats were intraperitoneally injected with 20％CCl4(once a week)according to body weight(5 mL/kg)to induce hepatic fibrosis for another 4 weeks.During CCl4 treatment,rats in the DLF group were also intraperitoneally injected with 25 mg/kg STZ(once a week).After STZ treatment,the contents of FBG,INS,HOMA-IR,and HbA1c were evaluated once a week.The level of FBG was detected by blood glucose meter,and INS and HbA1c contents were detected by ELISA.Biochemical analysis was used to analyze the serum levels of AST,ALT,Tbil,and HA,as well as the HYP content in liver tissues.HE and Masson staining were used to evaluate pathological changes in liver tissues.Results The contents of FBG,HbA1c,and HOMA-IR were significantly increased,while the INS content was significantly decreased in the DLF group compared with those in the normal or LF group.Compared with the normal group,the LF group and DLF group showed higher levels of ALT,AST,Tbil,HA,and HYP,as well as fibrotic changes and inflammation in the liver tissue.Conclusions HLSD combined with CCl4 and STZ inducement is an effective strategy for establishing a rat model of type 2 diabetes mellitus with liver fibrosis.
