Simultaneous determination of six alkaloid components in Zhachong Shisanwei Pills by QAMS
10.19778/j.chp.2025.02.011
- VernacularTitle:一测多评法同时测定蒙药扎冲十三味丸中6个生物碱成分
- Author:
Hongying BAO
1
;
Yukun ZHOU
;
Ziwei CHEN
;
Zengyun JI
;
He MENG
;
Junsheng HAO
;
Ying XIN
Author Information
1. 内蒙古民族大学蒙医药学院,内蒙古 通辽 028000
- Publication Type:Journal Article
- Keywords:
Mongolian medicine Zhachong Shisanwei Pills;
quantitative analysis of multi-components by single marker(QAMS);
relative correction factor;
benzoylmesaconine;
benz-oyl-hypaconine;
benzoylaconine;
mesaconi-tine;
hypaconitine;
aconitine
- From:
Drug Standards of China
2025;26(2):190-197
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quantitative analysis of multi-components by single marker(QAMS)for the determination of 6 alkaloid components,which is benzoylmesaconine,benzoyl-hypaconine,benzoylaconine,mesaconitine,hypaconitine,and aconitine in Zhachong Shisanwei Pills,and prove the scientificity and feasibility of the method in the quality analysis.Methods:The chromatographic separation was performed on an Agilent Eclipse Plus C18(250 mm×4.6 mm,5 μm)with gradient elution using 0.1 mol·L-1 ammonium acetate(0.5 mL of gla-cial acetic acid per 1 000 mL)(A)-acetonitrile:tetrahydrofuran(25∶15)(B),as the mobile phase(0-50 min,18%B-28%B),the detection wavelength was switched from 235 nm,the column temperature was kept at 40℃and the flow rate was 1.0 mL·min-1.The relative correction factors(fs/i)were established with the other 5 compo-nents to be measured using benzoylaconine as the internal reference,which were used to calculate the mass fraction of each component.At the same time,the mass fractions of the 6 effective constituents in Zhachong Shisanwei Pills were calculated by the external standard method(ESM).By comparing the content results of ESMand QAMS,the accura-cy of QAMS method were evaluated.Results:The relative correction factors(fs/i)of benzoylmesaconine,benzoylhyp-aconine,mesaconitine,hypaconitine,and aconitine in Mongolian medicine Zhachong Shisanwei Pills were reproduci-ble with good reproducibility,which were 0.680 4,0.450 6,0.850 8,0.676 1 and 0.757 0,the result obtained by QAMS approximated those obtained by external standard method(ESM).Conclusion:The method is simple,stable and reproducible,and can be used for the quality control of 6 alkaloid components in Zhachong Shisanwei Pills.
