Angelica polysaccharides improve diabetic retinopathy by promoting lncRNA MEG3 expression
10.13389/j.cnki.rao.2025.0019
- VernacularTitle:当归多糖通过促进lncRNA MEG3表达改善糖尿病视网膜病变
- Author:
Neng LI
1
;
Jian LAI
1
Author Information
1. 310007 浙江省杭州市,杭州市中医院眼科
- Publication Type:Journal Article
- Keywords:
angelica polysaccharide;
diabetic retinopathy;
lncRNA MEG3;
cellular pyroptosis
- From:
Recent Advances in Ophthalmology
2025;45(2):102-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of angelica polysaccharides on diabetic retinopathy(DR)and possi-ble mechanisms of action.Methods DR mouse models were established by injecting streptozotocin into 18 C57BL/6J mice.The mice were then divided randomly into an Angelica polysaccharide group(given 289 mg·kg-1 Angelica polysac-charides),a positive control group(given 217 mg·kg-1 calcium hydroxybenzenesulfonate),and a model group(given an equal volume of saline),6 mice in each group.6 normal mice were selected as a blank group(given an equal volume of sa-line).The drug was administered by gavage once a day for 28 consecutive days.ARPE-19 cells were exposed to a high-glu-cose(25 mmol·L-1)culture medium to establish DR cell models,which were divided into a negative control group(con-trol vector),a lncRNA MEG3 group(lncRNA MEG3 overexpression vector),and a model group(DR cells without vector transfection).Cells not treated with 25 mmol·L-1glucose were taken as a blank group.All cells were cultured for 24 h.Fasting blood glucose levels were detected in mice.Hematoxylin-Eosin(HE)staining was performed to study the his-topathological changes in the retinal tissue of mice.Quantitative polymerase chain reaction(qPCR)was performed to de-tect the expression level of lncMEG3 mRNAs in the retinal tissue of mice.CCK-8 assay was used to measure the activity of ARPE-19 cells.Enzyme linked immunosorbent assay(ELISA)was used to detect IL-1β and IL-6 levels in mouse retinal tis-sues and ARPE-19 cells.The Western blot analysis was made to detect the levels of cellular pyroptosis-related proteins(in-cluding Caspase-1,Cleaved-Caspase-1,GSDMD,GSDMD-N,NLRP3,IL-1 β,and IL-18)in mouse retinal tissues and ARPE-19 cells.Results Blood glucose levels in the mice of the model group were higher than those in the mice of the blank group and lower than those in the mice of Angelica polysaccharide and positive control groups(all P＜0.05).HE staining results showed that compared with those of the blank group,the mice in the mode group had significantly damaged retinal tissues and disordered and loose cell arrangement.The retinal tissue of the mice in Angelica polysaccharide and positive control groups was arranged more orderly and densely and suffered less damage,compared with that of the mice in the model group.The relative expression levels of lncMEG3 mRNAs in the retinal tissue of mice in the blank,model group,An-gelica polysaccharide,and positive control groups were 1.005±0 114,0.423±0.054,0.701±0.101 and 0.593±0.084,re-spectively.The retinal expression levels of lncMEG3 mRNAs in the mice of Angelica polysaccharide and positive control groups were significantly higher than those in the mice of the model group(both P＜0.01).CCK-8 results showed that the survival rates of ARPE-19 cells in the lncRNA MEG3 group at 48 and 72 h were higher than those in the negative control group(both P＜0.05).ELISA and Western blot results showed that the retinal expression levels of IL-1 β,IL-6 and cellular pyroptosis-related proteins in the mice of the model group were significantly higher than those in the mice of the blank group(all P＜0.01)and higher than those in the mice of Angelica polysaccharide group and positive control group(all P＜0.05).The expression levels of IL-1 β,IL-6,and cellular pyroptosis-related proteins in ARPE-19 cells of the model group were significantly higher than those in the blank group(all P＜0.01).The expression levels of IL-1 β,IL-6,and cellular py-roptosis-related proteins in ARPE-19 cells of the lncRNA MEG3 group were lower than those in the negative control group(all P＜0.05).Conclusion Angelica polysaccharides can improve DR by promoting lncRNA MEG3 expression and down-regulating IL-1 β,IL-6,Caspase-1,Cleaved-Caspase-1,GSDMD,GSDMD-N,NLRP3,IL-1β,and IL-18 protein ex-pression.
