Exploring the effects and mechanisms of electroacupuncture on cognitive function in rats with cerebral ischemia-reperfusion injury based on the steady-state of neurovascular units
10.3969/j.issn.1001-1242.2025.02.001
- VernacularTitle:基于神经血管单元稳态的电针对脑缺血再灌注损伤大鼠认知功能的影响及作用机制
- Author:
Ting RAO
1
;
Bai CHEN
;
Shufang LIN
Author Information
1. 福建中医药大学附属康复医院,福建省 福州市,350003;福建省康复技术重点实验室;福建中医药大学康复医学院
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion injury;
cognitive function;
electroacupuncture;
Baihui;
Shenting;
neu-rovascular unit
- From:
Chinese Journal of Rehabilitation Medicine
2025;40(2):161-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of electroacupuncture at Baihui and Shenting points on cognitive function in cerebral ischemia-reperfusion model rats and explore its mechanism.Method:Healthy male SD rats were randomly divided into Sham group,Model group,Electroacupuncture group,and Non-acupoint group(n=15 per group).Except for the Sham group,all other groups were prepared with a middle cerebral artery ischemia-reperfusion(MCAO/R)model.The Electroacupuncture group received 7 days of intervention with acupoints Baihui and Shenting,while the Non-acupoint group received 7 days of in-tervention with a 5mm outward of acupoints Baihui and Shenting.The Model group and Sham group only re-ceived the same conditions of grasping and fixation.Model testing was conducted using neurobehavioral scor-ing and TTC staining.Cognitive function was evaluated using neurobehavioral scoring,hippocampal cell mor-phology was observed using HE staining;CD31,GFAP,and IBa1 expression were observed using immunohis-tochemistry,and VEGF,BDNF,and IL-10 expression were observed using ELISA in the hippocampus.Result:Electroacupuncture at Baihui and Shenting acupoints can shorten the escape latency of Morris water maze in MCAO model rat,increase the number of crossing platforms,improve hippocampal cell edema,ne-crosis,and inflammatory cell infiltration,increase VEGF,IL-10,BDNF protein expression,increase CD31 positive expression,and reduce the number of GFAP and IBal positive cells.Conclusion:Electroacupuncture at Baihui and Shenting points can improve cognitive function in rats with cere-bral ischemia-reperfusion injury,and the mechanism of action may be related to regulating the components of the neurovascular unit(NVU)and maintaining NVU homeostasis.
