Determination of 32 anabolic agents in tablets,capsules and injections by liquid chromatography-high resolution mass spectrometry
10.3969/j.issn.1000-6710.2025.06.004
- VernacularTitle:液相色谱-高分辨质谱联用法测定药片、胶囊和注射液中32种蛋白同化制剂
- Author:
Yu LIU
1
;
Xianyu FAN
;
Zhenshuo GUO
;
Xiaoyu QIAN
;
Ping XIANG
;
Hui YAN
Author Information
1. 司法鉴定科学研究院,上海市法医学重点实验室,上海市司法鉴定专业技术服务平台,司法部司法鉴定重点实验室(上海 200063);昆明医科大学法医学院(昆明 650500)
- Publication Type:Journal Article
- Keywords:
anabolic androgenic steroids;
Stanozolol;
in vitro samples;
liquid chromatography-high resolution mass spectrometry
- From:
Chinese Journal of Sports Medicine
2025;44(6):451-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine 32 anabolic agents in tablets,capsules and injections using liquid chromatography-high resolution mass spectrometry(LC-HRMS),so as to provide technical support in the fields of anti-doping and judicial identification.Methods The tablet grinded into powder,capsule samples extracted using a methanol∶water(7∶3)solution and injection underwent centrifugation and filtration.The separation was performed on a Waters Acquity UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm),followed by gradient elution using ammonium acetate(20 mmol/L)solution containing 0.1%formic acid,5%acetonitrile and acetonitrile system as mobile phases,and detection by heated electrospray ionization in positive mode with a primary full-scan/data-dependent secondary scan mode.The established analytical method was subjected to methodological validation and parameters such as se-lectivity,sensitivity,matrix effect and delay effect were investigated.Meanwhile,the method was ap-plied to the detection of anabolic agents in 20 samples of tablets,capsules and injections provided by the authorities.Results The detection limits for 32 anabolic agents ranged from 1～200 μg/mg in the powder and 1～200 ng/mL in the injection.When this method was applied to the analysis of actual samples,five anabolic agents,including methandrostenolone and stanozolol,were identified among the 20 samples,disconsistent with labels on the package.Conclusion The LC-HRMS method is simple in pretreatment,sensitive and suitable for the detection of anabolic agents in tablet,capsule and injec-tion samples in the fields of anti-doping and judicial identification.
