Taohong Siwu Decoction Alleviates Neural Injury by Inhibiting NLRP3/caspase-1-Mediated Pyroptosis of Microglia
10.11969/j.issn.1673-548X.2025.06.011
- VernacularTitle:桃红四物汤通过抑制NLRP3/caspase-1介导的小胶质细胞焦亡减轻神经损伤的机制研究
- Author:
Xinru CHEN
1
;
Huifang WANG
;
Xian ZHOU
Author Information
1. 230012 合肥,安徽中医药大学药学院
- Publication Type:Journal Article
- Keywords:
Taohong Siwu decoction;
Microglia;
Pyroptosis;
NLRP3 inflammasome;
Oxygen glucose deprivation/reperfusion
- From:
Journal of Medical Research
2025;54(6):57-63
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Taohong Siwu Decoction(THSWD)in microglia pyroptosis mediated neuronal injury in PC12 under oxygen-glucose deprivation/reperfusion(OGD/R)and related mechanisms.Methods PC12 and BV2 cells were co-cultured using the OGD/R model to simulate ischemia-reperfusion injury of in vitro.The experiment was grouped as follows:control(PC12,PC12+BV2),OGD/R group(PC12,PC12+BV2),and OGD/R+10％THSWD-containing serum group(PC12,PC12+BV2).Oxygen and glucose was restored for 24h after 4-6h of deprivation.The severity of damage to PC12 cells was evaluated by cell counting kit-8(CCK-8)and flow cytometry.BV2 cells were used for the experiments and were divided into control,OGD/R and OGD/R+THSWD-containing serum(5％,10％,15％)groups,which were moulded and administered in the same way,and cell py-roptosis proteins proteinscysteinyl aspartate specific proteinase-1(caspase-1),apoptosis-associated speck-like protein containing a CARD(ASC),gasdermin D(GSDMD),interleukin-1β(IL-1β),interleukin-18(IL-18)and nucleotide-binding oligomeriza-tion domain-like receptor protein 3(NLRP3)inflammasome-associated proteins were detected by Western blot.Results In the pres-ence of BV2 cells,THSWD was able to further restore the morphology,increase cell viability(P＜0.05)and reduce apoptosis of PC12 cells after OGD/R injury(P＜0.05).In addition,THSWD was able to reduce the expression of OGD/R-induced pyroptosis proteins(caspase-1,ASC,GSDMD,IL-1β,IL-18)in BV2 microglia cells(P＜0.05).In contrast,the inhibitory effects of THSWD on BV2 cells pyroptosis proteins and NLRP3 inflammasome were reversed by NLRP3/caspase-1 agonist(P＜0.05).Conclusion THSWD protects PC 12 cells against OGD/R injury via inhibiting microglia pyroptosis mediated by the NLRP3/caspase-1 pathway.
