Q-marker prediction analysis of Sanqi Shenfeng oral liquid based on fingerprint and network pharmacology
10.12173/j.issn.1005-0698.202410109
- VernacularTitle:基于指纹图谱和网络药理学预测三七参蜂口服液的质量标志物
- Author:
Liping FENG
1
;
Weijun XIA
;
Jinyu LI
;
Dingqian ZHANG
;
Ping REN
;
Lihe LU
Author Information
1. 云南省药物研究所(昆明 650111);云南省中药和民族药新药创制企业重点实验室(昆明 650111)
- Publication Type:Journal Article
- Keywords:
Sanqi Shenfeng oral liquid;
Fingerprints;
Network pharmacology;
Quality marker
- From:
Chinese Journal of Pharmacoepidemiology
2025;34(1):35-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the fingerprints and predict the quality markers of Sanqi Shenfeng oral liquid based on fingerprint and network pharmacology.Methods The fingerprints of 12 batches of Sanqi Shenfeng oral liquid were established by using HPLC,and their peaks were identified and assigned.The candidate components were selected by multiple statistical analysis methods such as similarity evaluation,hierarchical cluster analysis,principal component analysis and orthogonal partial least squares discrimination analysis(OPLS-DA).The"component-target-pathway"network diagram was constructed by network pharmacology,and the quality markers of Sanqi Shenfeng oral liquid were predicted.Results The 13 common peaks were identified from the established fingerprint.Compared with the reference material,eight common peaks were identified as 3(tetrahydroxystilbene glucoside),5(sodium benzoate),6(lobetyolin),7(notoginsenoside Ri),9(ginsenoside Rgi),10(ginsenoside Re),12(10-hydroxy-2-decenoic acid),13(ginsenoside Rb1).The similarity of 12 batches of Sanqi Shenfeng oral liquid samples was higher than 0.997,and 12 batches of samples were grouped into two categories.OPLS-DA analysis showed that peaks 2,3,4,7,9,10,11,12 were the main signature components affecting the quality of Sanqi Shenfeng oral liquid.Network pharmacology predicted that lobetyolin,notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1,ginsenoside Re and 10-hydroxy-2-decenoic acid were potential Q-markers of Sanqi Shenfeng oral liquid.The traditional functions are performed through STAT3/AKT1-Drp1,HIF-1 and PI3K-AKT signaling pathway.Conclusion The established fingerprint has good reproducibility,stability and feasibility.The six components have great influence on the quality of Sanqi Shenfeng oral liquid,which are transferable and traceable,and are closely related to the efficacy.They can be used as potential quality markers to provide a scientific basis for the quality control and evaluation of Sanqi Shenfeng oral liquid.
