Study on the promotion of osteo/odontogenic differentiation on SCAPs and anti-inflammatory effects of EGCG/CeO2-loaded nanoparticles in an inflammatory microenvironment
10.13591/j.cnki.kqyx.2025.07.009
- VernacularTitle:EGCG/CeO2负载纳米颗粒在炎症微环境下对SCAPs的作用研究
- Author:
Yuting WU
1
;
Mingrui DAI
;
Diya LENG
;
Tingting ZHU
;
Tao WU
;
Daming WU
Author Information
1. 南京医科大学附属口腔医院牙体牙髓病科,江苏南京(210029);口腔疾病研究与防治国家级重点实验室培育建设点,江苏南京(210029);江苏省口腔转化医学工程研究中心,江苏南京(210029)
- Publication Type:Journal Article
- Keywords:
reactive oxygen species;
antioxidant;
apical papilla stem cells;
anti-inflammatory;
osteogenic differentiation
- From:
STOMATOLOGY
2025;45(7):529-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of composite nanoparticles E/Ce@MCSNs loaded with epigallocatechin-3-gallate(EGCG)and cerium dioxide(CeO2)on the odonto/osteogenic differentiation of human stem cells from the apical papilla(SCAPs)and macrophage polarization under inflammatory conditions.Methods E/Ce@MCSNs were synthesized and characterized.Cell viabil-ity of SCAPs and RAW 264.7 cells treated with varying concentrations of E/Ce@MCSNs was assessed via CCK-8 assay.The antioxidant enzyme-mimetic activity of E/Ce@MCSNs was evaluated.Under simulated inflammatory conditions,intracellular reactive oxygen species(ROS)scavenging capacity was measured via DCFH-DA fluorescent probe.Alkaline phosphatase(ALP)staining/activity,alizarin red staining/semi-quantitative analysis,and RT-qPCR were performed to detect odonto/osteogenic differentiation markers,in-cluding dentin sialoprotein(DSPP),ALP,runt-related transcription factor 2(Runx-2),type Ⅰ collagen(COL-Ⅰ),and osteopontin(OPN)in SCAPs.The effects of E/Ce@MCSNs on the odontogenic/osteogenic differentiation of SCAPs under this condition were eval-uated.RT-qPCR were used to analyze cytokine expression(TNF-α,IL-1β,IL-6,iNOS,TGF-β,IL-10)and secreted TNF-α/IL-6 levels in RAW264.7 cells.The concentrations of TNF-α and IL-6 in cell cul-ture supernatants were measured by ELISA.Results E/Ce@MCSNs exhibited excellent biocompatibility at concentrations≤100 μg/mL.They demonstrated potent ROS-scavenging activity(P＜0.05)and sig-nificantly enhanced ALP activity(P＜0.001),promoted calcium nodule formation(P＜0.001),and upregulated odonto/osteogenic gene expression(DSPP,ALP,Runx-2,COL-Ⅰ,OPN)in SCAPs under inflammatory conditions(P＜0.05).In RAW264.7 cells,E/Ce@MCSNs downregulated pro-inflammatory cytokines(TNF-α,IL-1 β,IL-6,iNOS)(P＜0.01)and upregulated anti-inflammatory factors(TGF-β,IL-10)(P＜0.001),while reducing TNF-α and IL-6 secretion(P＜0.001).Conclusion E/Ce@MCSNs exert antioxidant and anti-inflammatory effects in apical periodontitis by scavenging excessive ROS,thereby promoting odonto/osteogenic differentiation of SCAPs.
