Effect of rosa roxburghii Tratt polysaccharides on insulin resistance in gestational diabetes mellitus rats by regulating adenosine monophosphate-activated proteinkinase/peroxlsome proliferator-activated receptor-γ coactlvator-1α signaling pathway
10.3969/j.issn.1006-6187.2025.07.010
- VernacularTitle:刺梨多糖调节腺苷一磷酸活化蛋白激酶/过氧化物酶体增殖物活化受体γ共激活因子α信号通路改善妊娠期糖尿病大鼠胰岛素抵抗影响的研究
- Author:
Xiuping CHI
1
;
Jinling YAN
;
Wenhua CUI
;
Ling LIU
;
Lixuan WEN
Author Information
1. 050000 石家庄市第四医院产二科
- Publication Type:Journal Article
- Keywords:
Rosa roxburghii Tratt polysaccharides;
Adenosine monophosphate-activated protein kinase/peroxisome proliferator-activated receptor γ coactlvator-1α signaling pathway;
Gestational diabetes mellitus;
Insulin resistance
- From:
Chinese Journal of Diabetes
2025;33(7):536-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of rosa roxburghii Tratt polysaccharides(RRTP)on insulin resistance(IR)in rats with gestational diabetes mellitus(GDM)by regulating adenosine monophosphate-activated protein kinase(AMPK)/peroxlsome proliferator-activated receptor-γ coactlvator-1α(PGC-1α)signaling pathway.Methods Ten pregnant rats served as normal control(NC)group,and 50 GDM rats were randomly divided into GDM group,RRTP 100 mg/kg(RRTP 100)group,RRTP 200 mg/kg(RRTP 200)group,positive drug(PD)group,RRTP 200 mg/kg+Dorsomorphin(RRTP 200+Dorsomorphin)group.The indexes of glucose and lipid metabolism,superoxide dismutase(SOD),malondialdehyde(MDA),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and pathological changes were evaluated in each group,and Schmidt scoring shall be performed.Additionally,apoptosis and the expression of proteins related to the AMPK/PGC-1α pathway in tissues shall be detected.Results Fasting plasma glucose,fasting insulin,homeostatic model assessment-IR,total cholesterol,triglycerides,low-density lipoprotein cholesterol,MDA,IL-6,TNF-α,Schmidt score and apoptosis index were higher in GDM group than in NC group(P<0.05),while the expressions of high-density lipoprotein cholesterol,SOD,p-AMPK/AMPK,p-AMPK and PGC-1α protein were lower in GDM group than in NC group(P<0.05).The above indexes improved in RRTP 100,RRTP 200 and PD groups.In the RRTP 200+Dorsomorphin group,the ameliorative effect of RRTP on IR was reversed.Conclusions RRTP improve IR in GDM rats by activating the AMPK/PGC-1α signaling pathway.
