Effect of circular RNA polyribonucleotide nucleotidyltransferase 1 on glucose and lipid metabolism and offspring outcomes in rats with gestational diabetes mellitus through microRNA-345-3p/lipid raft scaffold protein 2 axis
10.3969/j.issn.1006-6187.2025.07.009
- VernacularTitle:环状RNA多核苷酸核苷酸转移酶1通过微小RNA-345-3p/脂筏特征蛋白2轴影响妊娠期糖尿病大鼠糖脂代谢和子代结局机制的研究
- Author:
Lingling TONG
1
;
Hongping LI
;
Jing ZHANG
;
Chunhui MA
;
Suxin CUI
;
Jing TAN
;
Wenhua CUI
Author Information
1. 050035 石家庄市第四医院产科
- Publication Type:Journal Article
- Keywords:
Circular RNA polyribonucleotide nucleotidyltransferase 1;
MicroRNA-345-3p/lipid raft scaffold protein 2 axis;
Gestational diabetes mellitus;
Glucose and lipid metabolism;
Offspring outcome
- From:
Chinese Journal of Diabetes
2025;33(7):528-535
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impacts and mechanism of circular RNA polyribonucleotide nucleotidyltransferase 1(Circ-PNPT1)on glucose and lipid metabolism and offspring outcomes in rats with GDM through microRNA-345-3p(miR-345-3p)/lipid raft scaffold protein 2(FLOT2)axis.Methods 75 pregnant female rats were divided into normal control(NC)group,GDM group,si-NC group,si-Circ-PNPT1 group,and si-Circ-PNPT1+miR-345-3p-inhibitor group,with 15 rats in each group.Glucose and lipid metabolism indexes were detected in each group.FIns was detected by ELISA.The survival rate and body weight of fetal rats were compared in each group.The expression of Circ-PNPT1,miR-345-3p and FLOT2 mRNA in placenta was detected by qRT-PCR.The double luciferase reporter gene experiment verified the targeting relationship between miR-345-3p and Circ-PNPT1 and FLOT2.Western blot was used to detect the expression of FLOT2 protein in rat placenta.Results Compared with the NC group,the level of FPG,FIns,HOMA-IR,TC,TG,LDL-C,embryo weight,and the expressions of Circ-PNPT1,FLOT2 in GDM group increased,the level of HDL-C,embryo survival rate and the expression of miR-345-3p decreased(P<0.05).Compared with GDM group,the level of FPG,FIns,HOMA-IR,TC,TG,LDL-C,embryo weight,and the expression of FLOT2 in si-Circ-PNPT1 group decreased,the level of HDL-C,embryo survival rate and the expression of miR-345-3p increased(P<0.05);MiR-345-3p-inhibitor reversed the improvement of si-Circ-PNPT1 on GDM rats(P<0.05).Conclusions Knocking down Circ-PNPT1 can up-regulate miR-345-3p and down-regulate FLOT2 to improve glucose and lipid metabolism and offspring outcome in GDM rats.
