Determination of sotagliflozin in rat plasma by UPLC-MS/MS method
10.13699/j.cnki.1001-6821.2025.02.016
- VernacularTitle:用UPLC-MS/MS法测定大鼠血浆中索格列净的浓度
- Author:
Ge-xi CAO
1
;
Xiao-xu ZHANG
;
Yan-ru DENG
;
Bin YAN
;
Zhan-jun DONG
Author Information
1. 河北医科大学 研究生学院,河北 石家庄 050051;河北省人民医院药学部,河北石家庄 050051
- Publication Type:Journal Article
- Keywords:
sotagliflozin;
plasma concentration;
pharmacokinetics;
ultra-high performance liquid chromatography-tandem mass spectrometer method
- From:
The Chinese Journal of Clinical Pharmacology
2025;41(2):230-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a ultra-high performance liquid chromatography-tandem mass spectrometer(UPLC-MS/MS)method for determining the concentration of sotagliflozin in rat plasma and apply it to pharmacokinetic studies in rats.Methods Electrospray negative ion multi-reaction ion detection was used.Chromatographic column:EXT-C18(2.1 mm × 100.0 mm,2.7 μm);column temperature:45 ℃;mobile phase:5 mmol·L-1 ammonium acetate aqueous solution-acetonitrile;flow rate:0.35 mL·min-1;ion pairs:sotagliflozin m/z 483.3→315.1,dapagliflozin m/z 467.4→329.2;injection volume:6 μL,plasma samples were processed using methyl tert-butyl ether liquid-liquid extraction.Six male SD rats were administered a single oral dose of sogliflozin at 40 mg·kg-1,and detected the concentration of sogliflozin in plasma.Pharmacokinetic parameters were calculated using Drug And Statistics(DAS)2.1.1.Results Sotagliflozin showed good linearity within the range of 5-2 000 ng·mL-1,with intra-day and inter-day precision both less than 15％.The recovery rate,matrix effect,and stability were all within the specified range.Pharmacokinetic parameters:Cmax was(3 716.67±568.28)ng·mL-1,tmax was(1.00±0.32)h,t1/2 was(2.28±0.45)h,AUC0-t was(1.70 × 104±2 075.87)ng·mL-1·h.Conclusion This study established a method for determining the concentration of sotagliflozin in rat plasma,which is characterized by high sensitivity,rapid detection,and good repeatability.It is suitable for the determination of sotagliflozin concentration in plasma and pharmacokinetic studies.
