Expression of WT1 Gene in Patients with Acute Myeloid Leukemia M2 and Its Impact on Prognosis
10.11969/j.issn.1673-548X.2025.01.012
- VernacularTitle:WT1基因在急性髓系白血病M2型患者中的表达及对其预后的影响
- Author:
Songqing QI
1
;
Yang LIU
;
Jie ZHU
Author Information
1. 830054 乌鲁木齐,新疆医科大学第一附属医院血液病中心;830054 乌鲁木齐,新疆医科大学医学科学研究所
- Publication Type:Journal Article
- Keywords:
WT1 gene;
Acute myelomonocytic leukemia;
AML1-ETO gene
- From:
Journal of Medical Research
2025;54(1):61-66,72
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level of WT1 gene in the bone marrow of patients with AML-M2 and its impact on the therapeutic effect and prognosis.Methods qRT-PCR was used to detect WT1 gene and AML1-ETO gene expression in bone marrow of 126 patients with AML-M2 and 30 control samples.The mutations of NPM1,FLT3-ITD/TKD,C-Kit8/17,DNMT3A and CEBPa were detected by Sanger gene sequencing method.Results The expression level of WT1 gene was significantly higher in patients with AML-M2 than the control group(0.0024 vs 0.080,P＜0.001).The level of WT1 gene expression was positively correlated with age(r=0.314,P=0.011)and the proportion of bone marrow original cells(r=0.534,P=0.010),the differences were statistically significant.The expression level of WT1 gene in normal karyotype group and abnormal karyotype group was significantly different(P=0.040).A comparison of AML1-ETO positive group and AML1-ETO negative group showed that the WT1 gene expression levels of the two groups were significantly different(P=0.032).The WT1 gene in the bone marrow of patients,who were AML1-ETO positive,with AML-M2 was positively correlated with the AML1-ETO gene expression level(r=0.524,P=0.037),and the AML1-ETO gene and WT1 gene expression levels were effectively tracked in 20 patients who werel AML1-ETO positive,with all positively correlationship(P＜0.05).There was no significant difference in the complete remission(CR)rate between WT1 gene high expression group and WT1 gene low expression group(66.7％vs 60.4％,P＞0.05).Both univariate analysis and multivariate COX analysis indicated that WT1 high expression group had higher overall survival(OS)rate(P＜0.05).Conclusion The expression level of WT1 gene in bone marrow may be used as a molecular index to monitor minimal residual disease(MRD),which has important clinical significance for prognosis as-sessment of AML-M2 patients.
