Screening and Exploring the Significance of Core Genes in Liver Cirrhosis Based on Bioinformatics
10.11969/j.issn.1673-548X.2025.01.011
- VernacularTitle:基于生物信息学对肝硬化中核心基因的筛选及意义探究
- Author:
Shijun SHEN
1
;
Xiang DENG
;
Yongfeng RAO
Author Information
1. 677099 临沧市人民医院肝胆胰微创外科
- Publication Type:Journal Article
- Keywords:
Cirrhosis;
Core genes;
Bioinformatics;
Clinical significance
- From:
Journal of Medical Research
2025;54(1):54-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the core genes in liver cirrhosis through bioinformatics screening,construct a network of their in-teractions,and explore the pathways and clinical significance of these genes.Methods Transcriptome datasets were obtained from the GEO database to screen for differential gene expression.GO analysis and KEGG pathway enrichment analysis were performed.A protein interaction network was constructed using the STRING database.The top 10 core genes were identified using the MCC algorithm,and the miRNA-mRNA regulatory relationships were established.The validation of the core gene expression was conducted with additional GEO datasets and clinical samples,and ROC analysis was used to evaluate the clinical significance.Results A total of 47 upregulated and 34downregulated genes were identified in this study.These differential genes were involved in biological processes such as extracellular matrix organization,urogenital system development,and cellular responses to copper,cadmium,and zinc ions.They performed the inter-action pathway function through the ECM receptor.The top 10 core genes were SPP1,SOX9,COL1A2,TAGLN,ACTA2,CCND1,CD24,VWF,JAG1 and MMP7.The expression of the core genes was increased in cirrhotic tissues,among which CCND1,CD24 and VWF showed high accuracy in distinguishing the cirrhotic tissues(AUC＞0.90).Conclusion The expression of the core genes in liver cirrhosis is increased and may lead to liver fibrosis through the ECM receptor interaction pathway,while the CCND1,CD24 and VWF could serve as effective targets for the diagnosis and treatment of liver cirrhosis.
