Establishment of animal model of blood stasis syndrome in diabetic kidney disease and metabonomic analysis
- VernacularTitle:糖尿病肾病血瘀证动物模型的建立及代谢组学分析
- Author:
Qian GUO
1
;
Sheng-fang WAN
;
Rong-ke LI
;
Lei ZHANG
;
Zhao-hui WEI
;
Zi-hui ZHONG
;
Jing SHAO
Author Information
- Publication Type:Journal Article
- Keywords: diabetic kidney disease; blood stasis syndrome; metabolomics; liquid chromatography-mass spectrometry technol-ogy; differential metabolites; metabolic pathways
- From: Chinese Pharmacological Bulletin 2025;41(3):592-599
- CountryChina
- Language:Chinese
- Abstract: Aim To establish an animal model of diabetic kidney disease(DKD)integrating blood stasis syndrome and syndrome evaluation indicators.Methods Twenty-five SD rats were ran-domly divided according to body weight into a control group(8 rats)and a modeling group(17 rats).The modeling group was fed a high-sugar and high-fat diet for four weeks and induced to form diabetic rats by intraperitoneal injection of 30 mg·kg-1 streptozocin.The modeling rats were randomly divided into the DKD group and blood stasis syndrome combination group accord-ing to 24-hour urinary protein(24-hUP).The blood stasis syn-drome combination group was induced to replicate the DKD blood stasis syndrome model by injecting 10％high molecular weight D-glucoside three times at a dose of 0.05 mg·kg-1 via tail vein.The model was evaluated based on random blood glu-cose level,24-hUP level,syndrome assessment,pathological staining etc,and differential metabolites were selected using metabolomics.Results The comprehensive evaluation of syn-drome manifestations and pathological staining in the combined model of blood stasis syndrome in rats demonstrated successful replication.Utilizing the technique of liquid chromatography-mass spectrometry,22 differential metabolites were identified,with associated pathways showing a certain relevance to blood stasis syndrome in DKD.Conclusions The successful replica-tion of an animal model combining the syndrome of blood stasis in DKD has been achieved in this study.Evaluation of indicators and results from metabolomics studies consistently demonstrate a correlation with the syndrome of blood stasis in DKD.
