Experimental Study on Ethanol Extract of the Root of Anacyclus Pyrethrum Regulates Gut Microbiota to Improve High-fat Diet-induced Dysglycemia in Mice
10.11969/j.issn.1673-548X.2025.01.020
- VernacularTitle:阿纳其根醇提取物调节肠道菌群改善高脂饮食诱导小鼠血糖紊乱的实验研究
- Author:
Hao YANG
1
;
GULIRUOYI·PAERHATI
;
Xiaoyue JIN
Author Information
1. 830011 乌鲁木齐,省部共建中亚高发病成因与防治国家重点实验室;830000 乌鲁木齐,新疆医科大学第六附属医院药学部(第六临床医学院药学教研室)
- Publication Type:Journal Article
- Keywords:
Ethanol extract of the root of anacyclus pyrethrum;
Gut microbiota;
High fat diet;
Obesity;
Dysglycemia
- From:
Journal of Medical Research
2025;54(1):106-110
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of ethanol extract of the root of anacyclus pyrethrum(EEAP)on blood glucose and gut microbiota in mice induced by high-fat diet.Methods Forty-eight male C57BL/6N mice were randomly allocated into groups using a random number table:control group,model group,low-,medium-,and high-dose EEAP groups,as well as a metformin group,with each group consisting of 8mice.Mice in the low,medium,and high dose EEAP groups were administered EEAP at 100,200 and 400mg/kg,respectively.The metformin group was given metformin at 280mg/kg.Both the control and model groups were given an equivalent a-mount of distilled water.Gavage was performed once daily for 12 consecutive weeks.Except the control group mice were fed normal diet,the other groups were fed high-fat diet.Fasting and random blood glucose were detected by blood glucose test strips.The levels of in-flammatory factors in mice were detected by enzyme-linked immunosorbent assay kit.Gut microbiota was detected by 16S rDNA real-time quantitative polymerase chain reaction.Results At 12th weeks,compared with the model group,the body mass of mice in low-dose,medium-dose and high-dose EEAP groups and metformin group were decreased significantly.Compared with the model group,the fasting blood glucose and random blood glucose in high-dose EEAP group and metformin group were significantly decreased.Com-pared with the model group,the abundations of Akkermansia muciniphila and Faecalibacterium prausnitzii in the intestinal tracts of mice in medium-dose and high-dose EEAP groups and metformin group were significantly increased,while the abundations of desulfovibrio were significantly decreased.Conclusion EEAP can improve dysglycemia induced by high-fat diet in mice,and its mechanism may be related to regulating gut microbiota and inhibiting inflammatory reaction.
