Oroxylin A induces apoptosis in Ishikawa cell line of endometrial cancer via PI3K/AKT signaling pathway
- VernacularTitle:千层纸素A通过PI3K/AKT信号通路诱导子宫内膜癌Ishikawa细胞株凋亡
- Author:
Huan-huan ZHAO
1
;
Yu-qian JIAO
;
Ruo-qi QIAO
;
Xue BAI
;
Na WANG
;
Yun-jie TIAN
;
Wen-ling FAN
;
Li LI
;
Su-wen SU
;
Yan FU
;
Hui ZHANG
;
Hong-fang YANG
Author Information
- Publication Type:Journal Article
- Keywords: Ishikawa cells,endometrial cancer; orox-ylin A; apoptosis; PI3K/AKT; CYP1B1; COMT
- From: Chinese Pharmacological Bulletin 2025;41(3):555-560
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of oroxylin A(OA)on apoptosis in Ishikawa cell line of endometrial cancer and the underlying mechanism through the phosphatidylinositol-3 kinase/protein kinase B(PI3K/AKT)signaling pathway.Methods Ishikawa cells were treated with different concentrations of OA(0,4,8,10,12,and 20 μmol·L-1)for 24 h-72 h,the cell viability was detected by CCK-8 assay,apoptosis was detected by flow cytometry,and the protein ex-pression levels of B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),PI3K/AKT,recombinant cytochrome P450 1B1(CYP1B1),and catechol-O-methyltransferase(COMT)were detected by Western blot technique.Results OA inhibited the prolifera-tion of Ishikawa cells in a concentration-and time-de-pendent manner.Compared with the blank control group,the expression of Bax protein increased signifi-cantly,while the expression of Bcl-2 protein decreased significantly with the increase of OA concentration.The expression of COMT protein increased significant-ly,while the expression of CYP1B1 protein decreased significantly.PI3K/AKT:IGF-1(PI3 K agonist)sup-plementation reversed the effect,the expression of COMT protein significantly decreased,and the expres-sion of CYP1B1 protein significantly increased.Con-clusions OA exerts anti-tumor effects in Ishikawa cells of endometrial cancer,which may be related to cell apoptosis mediated by the inhibition of the PI3K/AKT signaling pathway.
