Resveratrol inhibits H9c2 cardiomyocyte apoptosis induced by hypoxia/reoxygenation through activating JAK2/STAT3 signaling pathway
- VernacularTitle:白藜芦醇通过激活JAK2/STAT3信号通路抑制缺氧/复氧诱导的H9c2心肌细胞凋亡
- Author:
Jin-yu LI
1
;
Dan-mei HUANG
;
Yan-mei ZHANG
;
Fen-fei GAO
;
Bin WANG
Author Information
- Publication Type:Journal Article
- Keywords: resveratrol; hypoxia/reoxy genation; H9c2 cardiomyocytes; JAK2/STAT3 signaling pathway; cell apoptosis; AG490
- From: Chinese Pharmacological Bulletin 2025;41(3):451-456
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effects of resveratrol(RSV)on hypoxia/reoxygenation(H/R)-induced apoptosis in H9c2 cardiomyocytes and the un-derlying mechanisms.Methods A model of H/R was established in H9c2 cardiomyocytes.Cell apoptosis was determined by flow cytometry.The expressions of Bax,Bcl-2,cleaved caspase-3 and JAK2/STAT3 signa-ling pathway proteins were detected by Western blot.The inhibitor of JAK2,AG490,was used to assess the effect of RSV on H/R-induced apoptosis in H9c2 car-diomyocytes after inhibiting JAK2/STAT3 signaling pathway.Results RSV could effectively inhibit H/R-provoked cell apoptosis in H9c2 cardiomyocytes and increase the expression of p-JAK2 and p-STAT3.After AG490 intervention,the anti-apoptotic effect of RSV on H9c2 cardiomyocytes caused by H/R was significantly counteracted.Conclusions RSV can inhibit H/R-in-duced apoptosis in H9c2 cardiomyocytes through the activation of JAK2/STAT3 signaling pathway.
