Mining and Identifying Key Genes on the Biosynthetic Pathway of Indigo and Indirubin in Baphicacanthus cusia(Nees)Bremek
- VernacularTitle:马蓝中靛蓝和靛玉红生源合成途径上关键基因的筛选和验证
- Author:
Xiaoyu HAN
1
;
Song YANG
;
Chong FENG
;
Jianli YAN
;
Zhimin ZHAO
;
Depo YANG
Author Information
- Publication Type:Journal Article
- Keywords: Baphicacanthus cusia(Nees)Bremek.; Indigo; Indirubin; Biosynthetic pathway; DNA cloning by homologous recombination
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(7):2063-2077
- CountryChina
- Language:Chinese
- Abstract: Objective To study mine and identify the key genes on the biosynthetic pathway of indigo and indirubin in Baphicacanthus cusia(Nees)Bremek.(B.cusia).Methods A weighted co-expression network analysis of transcriptome and metabolome data was conducted to screen out candidate genes.Selected genes were further cloned by homologous recombination in Escherichia coli and Saccharomyces cerevisiae to confirm their function.The LC-MS analysis was used to test the metabolic products.Results The enzyme encoded by gene FMO-EVM0009245 recombined in Escherichia coli could oxidize indole to indigo and indirubin,While the enzyme encoded by gene CYP-EVM0022856 and CYP-EVM0028891 recombined in Saccharomyces cerevisiae could oxidize indole to indigo.Conclusion This article lays a foundation for further elucidating the molecular mechanism of indigo and indirubin biosynthesis and provided research basis for increasing the content of medicinal components of B.cusia.
