Bioinformatics analysis and experimental verification of disulfidptosis-related genes in vascular dementia
- VernacularTitle:血管性痴呆双硫死亡相关基因的生物信息学分析及实验验证
- Author:
Jin-zhi ZHANG
1
;
Wei CHEN
;
Gui-feng ZHUO
;
Er-wei HAO
;
Xiao-min ZHU
;
Yu-lan FU
;
Shan-shan PU
;
Ming-yang SU
;
Lin WU
Author Information
- Publication Type:Journal Article
- Keywords: vascular dementia; disulfidptosis; bioin-formatics; machine learning; experimental verification; differentially expressed genes
- From: Chinese Pharmacological Bulletin 2025;41(3):514-520
- CountryChina
- Language:Chinese
- Abstract: Aim To examine the pathogenesis of disul-fide death gene in vascular dementia(VD)by bioin-formatics analysis of disulfide death differentially ex-pressed genes(DEGs)combined with experimental verification.Methods The death DEGs of disulfide were screened and their correlation was analyzed.The VD patients data in the data set were analyzed by clus-tering and typing and gene set variation.The clustering risk of DEGs was tested with a nomogram model,and the optimal learning model was predicted.After the es-tablishment of VD rat model,water maze test,HE stai-ning and RT-qPCR detection were performed to verify the results of health information.Results Four DEGs including SLC7A11 were obtained,which had antago-nistic or synergistic interaction with each other.The genetic data could be divided into two subtypes with significant differences.After typing,VD disulfide DEGs were mainly concentrated in GnRH signaling pathways.The accuracy of the nomogram prediction model was high.Generalized linear was the best ma-chine learning model.Compared with the sham opera-tion group,the escape latency of rats in the model group was prolonged,the number of crossing platforms decreased,the relative mRNA expression levels of Slc3a2 and Slc7a11 decreased,and LRPPRC in-creased.Conclusions SLC7A11 and other disulfide death DEGs and its related GnRH signaling pathway may be an important part of the pathogenesis of VD di-sulfide death.SLC3A2,LRPPRC and SLC7A11 can be used as characteristic genes in the regulation of VD by disulfide death,which may affect VD progression through the regulation of disulfide death.
