Effect of rosavin on hepatocellular steatosis and its underlying mechanism
10.12360/CPB202404051
- VernacularTitle:络赛维对肝细胞脂肪变性的影响及机制
- Author:
Shen WANG
1
;
Jin-hui CAI
;
Lin ZHENG
;
Yan ZHANG
;
Kai-qing ZENG
;
Qi-en XU
;
Yan-min FENG
;
Xiao-xia YE
Author Information
1. 广东医科大学药学院,广东湛江 524023
- Publication Type:Journal Article
- Keywords:
non-alcoholic fatty liver disease;
Rhodiola rosea L.;
rosavin;
hepatocellular steatosis;
TNF-α;
PPARG
- From:
Chinese Pharmacological Bulletin
2025;41(3):466-474
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of rosavin on hepatocellular steatosis and its mechanism of action.Methods AML-12 and HepG2 cells were induced to undergo hepatocellular steatosis by free fatty acids(FFA),and the optimal inducing concentration was determined by oil red O staining and CCK-8 assay.The cell activity was detected by CCK-8 assay after ro-savin treatment,and the lipid droplet accumulation was observed by oil red O staining.The levels of triglycer-ide(TG),total cholesterol(TC),glutamic oxalacetic transaminase(AST),glutamic pyruvic transaminase(ALT),superoxide dismutase(SOD),glutathione per-oxidase(GSH-Px),and malondialdehyde(MDA)were detected by kits.The potential targets of rosavin in non-alcoholic fatty liver disease(NAFLD)were ana-lyzedby network pharmacology and molecular docking,and the expression of core candidate targets before and after the rosavin intervention was detected by real-time fluorescence quantitative PCR.Results Hepatocyte steatosis was induced by FFA,and the intervention of rosavin(25,50 μmol·L-1)reduced the number of intracellular lipid droplets in hepatocytes in a dose-de-pendent manner,also lowered the cellular levels of TG,TC,AST,ALT,elevated the levels of SOD and GSH-Px,and reduced the levels of MDA.Network pharma-cological analysis and molecular docking yielded five core candidate targets:NOS3,MAPK14,PPARG,TNF-α,and IGF-1,and real-time fluorescence quantitative PCR showed that the action of loxavir significantly re-duced the gene expression of TNF-α and PPARG in hepatocytes after FFA induction.Conclusions Rosa-vin can attenuate the inflammatory response,oxidative stress level,and lipid accumulation in hepatocytes by modulating TNF-α and PPARG,thereby ameliorating FFA-induced hepatocellular steatosis.
