Effect of SOCS3 Overexpression on CD4+ T cells Imbalance in Rats with Experimental Autoimmune Myocarditis
10.11969/j.issn.1673-548X.2025.06.021
- VernacularTitle:SOCS3过表达对实验性自身免疫性心肌炎大鼠CD4+ T细胞失衡的影响
- Author:
Xu ZHANG
1
;
Feng ZHU
1
;
Lingqin WU
1
Author Information
1. 314000 嘉兴市第二医院(嘉兴大学附属第二医院)儿科
- Publication Type:Journal Article
- Keywords:
Autoimmune myocarditis;
Suppressors of cytokine signaling 3;
Janus kinase 2/signal transducer and activator of tran-scription 3signal pathway;
CD4+T cells
- From:
Journal of Medical Research
2025;54(6):114-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and mechanisms of suppressor of cytokine signaling 3(SOCS3)overexpression on CD4+T cells imbalance in rats with experimental autoimmune myocarditis(EAM).Methods Ten male Lewis rats were randomly select-ed as the control group from 30male Lewis rats.The remaining 20 rats were injected with porcine cardiac myosin on the 0th and 7th days of the experiment to establish the EAM rat model.These 20 rats were then randomly divided into two groups:the EAM group and the EAM+SOCS3group.The EAM+SOCS3group was injected with SOCS3-overexpressing lentivirus via the tail vein,while the EAM group was injected with an equal volume of empty vector lentivirus.All rats underwent echocardiography to evaluate cardiac function and were then euthanized on the 21st day of the experiment.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum cardiac troponin T,creatine kinase-myocardial band,B-type natriuretic peptide,and cytokine levels.Myocardial tissue inflam-mation infiltration and myocardial pathological scores were evaluated by hematoxylin-eosin(HE)staining.The levels of CD4+T cell subsets in the rat spleen were detected by flow cytometry.The expressions of SOCS3,Janus kinase 2(JAK2),signal transducer and acti-vator of transcription 3(STAT3),and CD4+T cell-specific transcription factors were detected by real time fluorescent quantitative poly-merase chain reaction(RT-qPCR)and Western blot.Results In the EAM group,the levels of serum of cardiac troponin T,creatine kinase-myocardial band,and B-type natriuretic peptide,were significantly increased compared with those in the control group(P＜0.05),and the myocardial pathological score was significantly increased(P＜0.05);In the EAM group,the mRNA and protein expres-sions of T-box expressed in T cells(T-bet)and retinoid-related orphan receptor γt(RORγt)in myocardial tissues were significantly increased compared with those in the control group(all P＜0.05),while the expression of forkhead box P3(Foxp3)was significantly de-creased(P＜0.05);In the EAM group,the percentages of helper T cell 1(Th1)and helper T cell 17(Th17)in the spleen were signif-icantly increased compared with those in the control group(both P＜0.05),and the percentage of regulatory T cell(Treg)was signifi-cantly decreased(P＜0.05);In the EAM group,the mRNA and protein expressions of JAK2 and STAT3 in myocardial tissues were signifi-cantly increased compared with those in the control group(P＜0.05).These indexes in the EAM+SOCS3group were significantly restored compared with those in the EAM group(P＜0.05).Conclusion SOCS3 overexpression can ameliorate the imbalance of CD4+T cells and alleviate myocardial damage in EAM rats,and its mechanism is related to the downregulation of the JAK2/STAT3signaling pathway.
