Effects of platelet-derived growth factor-DD on proliferation and multilineage differentiation of human tendon-derived stem cells
- VernacularTitle:血小板衍生生长因子DD对人肌腱源性干细胞增殖和多向分化能力的影响
- Author:
Huawei WEN
1
;
Qingsong ZHANG
1
;
Ming TANG
1
;
Yanan LI
1
;
Hongfei TAN
1
;
Yushun FANG
1
Author Information
- Publication Type:Journal Article
- Keywords: tendon stem cell; tendon-derived stem cell; platelet-derived growth factor-DD; rotator cuff injury; rotator cuff repair; engineered stem cell
- From: Chinese Journal of Tissue Engineering Research 2025;29(31):6649-6655
- CountryChina
- Language:Chinese
- Abstract: BACKGROUND:Chronic rotator cuff injury is often companied by tendon degeneration and impaired function of tendon-derived stem cells.As am important cytokine,platelet-derived growth factor-DD has a regulatory effect on the proliferation and differentiation of tendon-derived stem cells.OBJECTIVE:To investigate the effect of platelet-derived growth factor-DD on the proliferation and multilineage differentiation of tendon-derived stem cells in human chronic rotator cuff injury.METHODS:Tendon-derived stem cells were isolated from human chronic rotator cuff injury tissue and cultured in vitro.Immunofluorescence staining was used to observe the cytoskeletal morphology of tendon-derived stem cells.Flow cytometry was used to identify the phenotype of tendon-derived stem cells.Tendon-derived stem cells were divided into two groups.The control group did not receive any intervention.The platelet-derived growth factor-DD group was treated with 5 μg/mL platelet-derived growth factor-DD.The effect of platelet-derived growth factor-DD on the proliferation and multilineage differentiation of tendon-derived stem cells was evaluated by cell proliferation assay and three-lineage differentiation assay.RESULTS AND CONCLUSION:(1)The number of EdU-positive cells in the platelet-derived growth factor-DD group was significantly increased compared with the control group(P＜0.05).Tendon-derived stem cells entered the rapid proliferation phase earlier,and the cell growth was logarithmic.(2)The positive areas of Oil Red O staining,Alcian Blue staining,and Alizarin Red staining in the platelet-derived growth factor-DD group were significantly larger than those in the control group(P＜0.05).(3)The above results show that platelet-derived growth factor-DD significantly promotes the proliferation and adipogenic,osteogenic,and chondrogenic differentiation of tendon-derived stem cells.
