A novel ROS-responsive nanocarrier for antioxidant therapy in endodontic inflammation
10.13591/j.cnki.kqyx.2025.07.006
- VernacularTitle:ROS响应型纳米载体用于牙髓炎症的抗氧化治疗
- Author:
Mingxin WANG
1
;
Yiyuan DUAN
1
;
Kejia FENG
1
;
Chenyu NIE
1
;
Qian MA
1
Author Information
1. 南京医科大学附属口腔医院综合诊疗科,口腔疾病研究与防治国家级重点实验室培育建设点,江苏省口腔转化医学工程研究中心,江苏南京(210029)
- Publication Type:Journal Article
- Keywords:
Ce-MBGNs;
dental pulp stem cells;
immunomodulation;
osteogenic/odontoblastic differentiation
- From:
STOMATOLOGY
2025;45(7):511-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of cerium-doped mesoporous bioactive glass nanoparticles(Ce-MBGNs)as reactive oxy-gen species(ROS)-responsive nanocarriers on inflammation in pulp and their potential to promote odontogenic differentiation of dental pulp stem cells(DPSCs).Methods The cytotoxicity of Ce-MBGNs was assessed by CCK-8.The expression of mRNA of osteogenic/odontogenic differentiation in DPSCs and that of inflammatory factor in RAW264.7 cells were detected by RT-qPCR.Alkaline phospha-tase and Alizarin Red S staining were utilized to investigate their ability to promote dentin mineralization.The ability of Ce-MBGNs to scavenge ROS was evaluated by immunofluorescence and JC-1 staining was used to assess the membrane potential(MMP)of mitochon-drial.Results Ce-MBGNs exhibited good biocompatibility and significantly increased the expression of osteogenic/odontogenic-related genes in DPSCs,promoting mineralization.Additionally,Ce-MBGNs effectively scavenged intracellular ROS,maintained MMP,inhib-ited the expression of pro-inflammatory factors,and promoted the expression of anti-inflammatory factors.Conclusion Ce-MBGNs can protect DPSCs from oxidative damage by maintaining the MMP of mitochondrial and controlling inflammation,and promote their odonto-blastic differentiation,providing a theoretical basis for the development of novel therapeutic agents for oral treatment.
