Calcined deer antler slices promote proliferation of bone marrow mesenchymal stem cells
10.12307/2025.673
- VernacularTitle:鹿角片煅制品促进骨髓间充质干细胞的增殖
- Author:
Xuekun SHAO
1
;
Dianhua SHI
;
Zhiping DING
;
Zhuoya QIU
;
Ping WANG
;
Yi WANG
;
Cheng WANG
;
Xiaoyan DING
;
Tiefeng SUN
Author Information
1. 山东中医药大学,山东省济南市 250355
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cell;
cell proliferation;
antler slice;
calcined;
yellow clay;
honey roasting;
water extract;
engineered stem cell
- From:
Chinese Journal of Tissue Engineering Research
2025;29(31):6601-6608
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Through scientific research addressing the effect of calcined deer antler slices on promoting the proliferation of bone marrow mesenchymal stem cells,it aims to provide empirical support for the integration and innovation of traditional Chinese medicine and modern regenerative medicine,and promote the widespread application of traditional Chinese medicine in the treatment of skeletal system diseases.OBJECTIVE:To investigate the effect of calcined deer antler slices on bone marrow mesenchymal stem cell proliferation.METHODS:Different calcination samples were prepared by wrapping deer antler slices with materials such as clay,yellow clay,and salted yellow clay,resulting in seven different samples(clay-cotton cloth,yellow clay-cotton cloth,salted yellow clay-cotton cloth,yellow clay-tin foil,salted yellow clay-tin foil,yellow clay-honey roasted,salted yellow clay-honey roasted antler slices).Water-soluble extract content in deer antler slices was determined before and after calcination.CCK-8 assay was used to evaluate the effects of different aqueous extracts of calcined antler slices on the proliferation activity of bone marrow mesenchymal stem cells.RESULTS AND CONCLUSION:(1)Calcination significantly increased the water-soluble extract content of deer antler slices,with the highest content observed in samples treated with yellow clay and honey.(2)Calcined deer antler slices significantly promoted bone marrow mesenchymal stem cell proliferation,among which the yellow clay-honey roasted deer antler slices have the most significant effect on promoting the proliferation of bone marrow mesenchymal stem cells.
