Changes in peripheral blood and bone marrow erythroblasts in rats after hypoxic exposure
- VernacularTitle:低氧暴露后大鼠外周血及骨髓红系的变化
- Author:
Hua XIONG
1
;
Jinjie LI
1
;
Lu WANG
1
;
Jie MA
1
Author Information
- Publication Type:Journal Article
- Keywords: hypoxia; rat; peripheral blood; erythrocyte; bone marrow; erythroblast
- From: Chinese Journal of Tissue Engineering Research 2025;29(25):5390-5395
- CountryChina
- Language:Chinese
- Abstract: BACKGROUND:The changes in peripheral blood erythrocytes of rats exposed to hypoxia are related to changes in proliferation of erythroblasts in the bone marrow.Related regulatory factors such as hypoxia-inducible factor and erythropoietin are involved.OBJECTIVE:To explore the changes and related factors of peripheral blood erythrocytes and bone marrow erythroblasts in rats after hypoxic exposure.METHODS:An altitude of 5 000 meters was simulated to establish an experimental rat model under a low-pressure and low-oxygen environment.The control group was raised in a laboratory at an altitude of 2 260 meters.The blood routine,bone marrow erythroblast ratio,hypoxia-inducible factor,and erythropoietin of rats were compared before and after hypoxic exposure.RESULTS AND CONCLUSION:(1)The peripheral blood erythrocyte count,hemoglobin,hematocrit and other indicators of the experimental group rats were higher than those of the control group(P＜0.05).(2)The proportion of bone marrow CD71+erythroblasts of the experimental group rats increased compared with the control group(P＜0.05).(3)The expression level of hypoxia-inducible factor-2α in bone marrow CD71+erythroblasts of experimental group rats increased compared with the control group(P＜0.05).(4)The erythropoietin level in the bone marrow supernatant of the experimental group rats was higher than that of the control group(P＜0.05).The results indicate that the proportion of peripheral blood erythrocytes and bone marrow erythroblasts in rats increases after hypoxic exposure,which may be related to the increased levels of hypoxia-inducible factor-2α in bone marrow erythroblasts and erythropoietin in bone marrow supernatant.
